Hematopathology Service, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Amyloid. 2021 Mar;28(1):35-41. doi: 10.1080/13506129.2020.1811962. Epub 2020 Sep 1.
Lichen or macular localised cutaneous amyloidoses have long been described as keratinic amyloidoses and believed to be due to the deposition of cytokeratin peptides originating from epidermis in the dermal papillae. However, recently it was suggested that galectin-7 is the causative protein for this type of amyloidosis. This was based on the detection of galectin-7 in a biopsy from a patient diagnosed with Bowen's disease and localised cutaneous amyloidosis. In this study we report mass spectrometry-based proteomic analysis of the protein composition of localised cutaneous amyloid deposits from seven patients using laser microdissection and show that basal keratins are the main constituents of the amyloid deposits. Galectin-7 was not present in the dermal amyloid deposits and was only present in the overlying Congo red negative epidermis.
长期以来，人们一直将苔藓样或斑状局限性皮肤淀粉样变描述为角质淀粉样变，并认为其是由于表皮来源的细胞角蛋白肽在真皮乳头沉积所致。然而，最近有研究表明半乳糖凝集素-7 是这种淀粉样变性的致病蛋白。这一观点基于对一位被诊断为 Bowen 病和局限性皮肤淀粉样变患者的活检中发现半乳糖凝集素-7 而提出。在本研究中，我们采用激光显微切割，对 7 位局限性皮肤淀粉样变患者的皮损进行基于质谱的蛋白质组学分析，并显示基底角蛋白是淀粉样沉积物的主要成分。半乳糖凝集素-7 不存在于真皮淀粉样沉积物中，而仅存在于刚果红阴性的表皮上层。
