Department of Clinical Medical College, Ningxia Medical University, Yinchuan.
Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou 730000, People's Republic of China.
Eur J Cancer Prev. 2021 Mar 1;30(2):154-160. doi: 10.1097/CEJ.0000000000000619.
China has a high incidence rate and low survival rate of gastric cancer. Therefore, there is a great need to further identify novel oncogenes and clinically applicable molecular targets for the diagnosis and treatment of this disease.
Expressions of PRRX1, Smad2, epithelial phenotype marker E-cadherin, and interstitial phenotype vimentin protein in a sample of 64 gastric carcinoma and adjacent nontumorous tissues were detected by immunohistochemistry. Their relationship and correlations with clinicopathological features were analyzed.
The positive rates of PRRX1, Smad2, E-cadherin, and vimentin protein in primary tumors were 60.94% (39/64), 59.38% (38/64), 34.38%(22/64), and 64.06% (41/64), respectively. A significant correlation was found among the expression of PRRX1, Smad2, E-cadherin, and vimentin (P < 0.05). Expression of the PRRX1, Smad2, and vimentin protein in gastric cancer tissue was correlated with Borrmann classification, lymph node-positive number, the degree of differentiation, depth of tumor invasion, and serum pepsinogen I (PGI) level (P < 0.05), but not with age, sex, serum carcinoembryonic antigen, serum CA199, or PGI/PGII (P > 0.05).
The positive rate of PRRX1 protein expression was positively correlated with the protein expression of Smad2 and vimentin, but negatively correlated with E-cadherin protein. PRRX1, Smad2, and vimentin proteins are associated with Borrmann type, lymph node positives, histologic grade, depth of tumor invasion, and serum PGI levels, all of which contribute to a poor prognosis for patients with gastric cancer.
中国胃癌的发病率高，生存率低。因此，非常有必要进一步鉴定新的癌基因和临床上可应用的分子靶点，用于胃癌的诊断和治疗。
采用免疫组织化学方法检测 64 例胃癌及癌旁组织中 PRRX1、Smad2、上皮表型标志物 E-钙黏蛋白和间质表型波形蛋白的表达，分析其与临床病理特征的关系及相关性。
64 例胃癌原发灶中 PRRX1、Smad2、E-钙黏蛋白和波形蛋白蛋白阳性率分别为 60.94%（39/64）、59.38%（38/64）、34.38%（22/64）和 64.06%（41/64）。PRRX1、Smad2、E-钙黏蛋白和波形蛋白表达之间存在显著相关性（P<0.05）。PRRX1、Smad2 和波形蛋白在胃癌组织中的表达与 Borrmann 分型、淋巴结阳性数、分化程度、肿瘤浸润深度和血清胃蛋白酶原 I（PGI）水平有关（P<0.05），但与年龄、性别、血清癌胚抗原、血清 CA199 或 PGI/PGII 无关（P>0.05）。
PRRX1 蛋白表达阳性率与 Smad2 和波形蛋白蛋白表达呈正相关，与 E-钙黏蛋白蛋白表达呈负相关。PRRX1、Smad2 和波形蛋白与 Borrmann 分型、淋巴结阳性、组织学分级、肿瘤浸润深度和血清 PGI 水平相关，均提示胃癌患者预后不良。
