Department of Genetics, NARIC, Agricultural Biotechnology Institute, Gödöllő, Hungary.
Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
FEBS Lett. 2020 Nov;594(21):3504-3517. doi: 10.1002/1873-3468.13918. Epub 2020 Sep 12.
Eukaryotic release factor 1 (eRF1) is a translation termination factor that binds to the ribosome at stop codons. The expression of eRF1 is strictly controlled, since its concentration defines termination efficiency and frequency of translational readthrough. Here, we show that eRF1 expression in Neurospora crassa is controlled by an autoregulatory circuit that depends on the specific 3'UTR structure of erf1 mRNA. The stop codon context of erf1 promotes readthrough that protects the mRNA from its 3'UTR-induced nonsense-mediated mRNA decay (NMD). High eRF1 concentration leads to inefficient readthrough, thereby allowing NMD-mediated erf1 degradation. We propose that eRF1 expression is controlled by similar autoregulatory circuits in many fungi and seed plants and discuss the evolution of autoregulatory systems of different translation termination factors.
真核释放因子 1（eRF1）是一种翻译终止因子，可与核糖体结合在终止密码子上。eRF1 的表达受到严格控制，因为其浓度决定了终止效率和翻译通读的频率。在这里，我们表明，Neurospora crassa 中的 eRF1 表达受自动调节回路控制，该回路依赖于 erf1 mRNA 的特定 3'UTR 结构。erf1 的终止密码子上下文促进通读，从而保护 mRNA 免受其 3'UTR 诱导的无意义介导的 mRNA 降解（NMD）。高浓度的 eRF1 导致通读效率降低，从而允许 NMD 介导的 erf1 降解。我们提出，许多真菌和种子植物中的 eRF1 表达受类似的自动调节回路控制，并讨论了不同翻译终止因子的自动调节系统的进化。
