Chemokine receptors on peripheral blood T lymphocytes in children on peritoneal dialysis.

BACKGROUND

Immune cell dysfunction is listed among complications resulting from chronic kidney disease (CKD). It could be associated with T-cells, which play a role in the lymphocytic migration and infiltration. However, the data on chemokine receptors expression on T-cells in patients with CKD particularly treated with peritoneal dialysis (PD) are still limited.

METHODS

The study aimed at multiparameter flow-cytometric analysis of the absolute numbers and percentage of T-cell subsets with surface chemokine receptors (CCR4, CCR5, CCR7, CXCR3, and CXCR4) or receptors' combinations in 47 children treated with PD.

RESULTS

We found lower absolute numbers of total T lymphocytes, lymphocytes with surface CCR5, CXCR4CCR5, CXCR3CCR5 antigens and T-cells with CCR4, CCR4CD4, CXCR3, CXCR3CD4, and CD8 receptors. Lymphocytes T with CD4, CCR7, CD28CCR7, CXCR3CD8 antigens showed higher percentage in children on PD as compared to healthy children and opposite percentage values of CCR4, CCR4CD4, CXCR3 T lymphocytes were diminished. Mean fluorescent intensity for CCR7, CCR7CD45RO, CCR7CD28, CXCR4CD4, CCR5CD4, CCR4, CCR4CD4 T-cells was lower in the PD group than in healthy children. The analysis of correlation between T lymphocyte subpopulations with chemokine receptors and other parameters revealed positive correlation of CCR7 and CCR7CD28 T-cells and weekly creatinine clearance, negative correlation between the percentage of CD45ROCCR7 antigen positive T-cells and KT/V.

SUMMARY

In conclusion, we could not confirm the phenomenon of earlier senescence of T-cells in children with CKD on PD treatment. This still requires further investigation. The higher percentage of T-cells with CCR7 surface receptor could be responsible for the increase of proliferation activity in this group of children.