Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, China.
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
J Sci Food Agric. 2021 Mar 15;101(4):1589-1597. doi: 10.1002/jsfa.10778. Epub 2020 Sep 15.
Ginkgotoxin including 4'-O-methylpyridoxine (MPN) and MPN-5'-glucoside (MPNG) is responsible for Ginkgo seed food poisoning. The purpose of the work reported was to prepare detoxified Ginkgo seed powder and at the same time to retain the nutritional and functional components of Ginkgo seed powder to the maximum extent.
Resin adsorption technology was firstly employed to remove ginkgotoxin from water extract of Ginkgo seed powder. Under optimal conditions, the adsorption efficiency of the optimal resin for MPN could reach 100%, and that for MPNG could only reach 85.4 ± 0.93%. Resin adsorption alone could not effectively remove MPN and MPNG simultaneously. Endogenous enzymatic hydrolysis was next attempted to transform MPNG to MPN. MPNG could be completely hydrolyzed to MPN by endogenous enzyme(s) at 40 °C and pH 5.0 in 180 min. Ginkgotoxin only in the form of MPN in the enzymatic hydrolysate was then adsorbed with resin and the conditions were statistically optimized. The adsorption efficiency of MPN reached 98.89 ± 0.99% under the optimized conditions.
Removal of ginkgotoxin by combining endogenous enzymatic hydrolysis with resin adsorption could preserve the main nutritional and functional components of Ginkgo seed powder to the most extent, and did not change its main characteristics. The ginkgotoxin removal method developed in this work is a relatively simple and efficient approach. © 2020 Society of Chemical Industry.
银杏毒素包括 4′-O-甲基吡哆醇（MPN）和 MPN-5′-葡萄糖苷（MPNG），是导致银杏种子食物中毒的原因。本工作的目的是制备脱毒银杏种子粉，同时最大限度地保留银杏种子粉的营养和功能成分。
首次采用树脂吸附技术从银杏种子粉水提液中去除银杏毒素。在最佳条件下，最优树脂对 MPN 的吸附效率可达 100%，而对 MPNG 的吸附效率仅为 85.4±0.93%。单独使用树脂吸附并不能有效地同时去除 MPN 和 MPNG。接下来尝试利用内源性酶水解将 MPNG 转化为 MPN。在 40°C 和 pH 5.0 条件下，内源性酶可将 MPNG 完全水解为 MPN，180 min 内水解率可达 100%。然后将仅以 MPN 形式存在于酶解液中的银杏毒素用树脂吸附，并对条件进行了统计学优化。在优化条件下，MPN 的吸附效率可达 98.89±0.99%。
将内源性酶解与树脂吸附相结合去除银杏毒素，可以最大限度地保留银杏种子粉的主要营养和功能成分，且不改变其主要特性。本工作开发的银杏毒素去除方法较为简单高效。 © 2020 英国化学学会。
