Department of Ophthalmology, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, 830000, China.
Acta Biochim Pol. 2020 Sep 1;67(3):373-378. doi: 10.18388/abp.2020_5228.
Diabetic retinopathy (DR), as the most frequent microvascular complication of diabetes mellitus (DM), causes vision loss and blindness in adults worldwide with increasing incidence. MicroRNAs (miRNAs) are involved in the regulation of DR. However, the role of miR-542-5p is still unknown. Here, we demonstrate that miR-542-5p is down-regulated in patients with DR and in high-glucose (HG)-treated retinal pigment epithelial cells. Moreover, miR-542-5p overexpression inhibits apoptosis in retinal pigment epithelial cells exposed to HG. The interaction between miR-542-5p and co-activator-associated arginine methyltransferase 1 (CARM1) is confirmed. MiR-542-5p mimics decrease the CARM1 level and miR-542-5p inhibitor increases the CARM1 level. Additionally, CARM1 overexpression promotes the miR-542-5p-mediated apoptosis in HG-treated retinal pigment epithelial cells. In summary, the data suggest that miR-542-5p may suppress apoptosis in retinal pigment epithelial cells via targeting CARM1, which provides a new therapeutic target for the treatment of patients with DR.
糖尿病性视网膜病变（DR）是糖尿病（DM）最常见的微血管并发症，其发病率在全球范围内不断上升，导致成年人视力丧失和失明。微小 RNA（miRNA）参与了 DR 的调控。然而，miR-542-5p 的作用尚不清楚。在这里，我们证明 miR-542-5p 在 DR 患者和高糖（HG）处理的视网膜色素上皮细胞中下调。此外，miR-542-5p 的过表达抑制了暴露于 HG 中的视网膜色素上皮细胞的凋亡。证实了 miR-542-5p 与共激活剂相关精氨酸甲基转移酶 1（CARM1）之间的相互作用。miR-542-5p 模拟物降低了 CARM1 水平，而 miR-542-5p 抑制剂增加了 CARM1 水平。此外，CARM1 的过表达促进了 HG 处理的视网膜色素上皮细胞中 miR-542-5p 介导的凋亡。总之，数据表明，miR-542-5p 可能通过靶向 CARM1 抑制视网膜色素上皮细胞的凋亡，为 DR 患者的治疗提供了新的治疗靶点。
