Opt Lett. 2020 Sep 1;45(17):4859-4862. doi: 10.1364/OL.398940.
We develop a multimodal imaging platform, combining depth-resolved scattering contrast from spectral-domain optical coherence tomography (SD-OCT) with complementary, non-contact absorption contrast using photoacoustic remote sensing (PARS) microscopy. The system provides a widefield OCT mode using a telecentric scan lens, and a high-resolution, dual-contrast mode using a 0.26 numerical aperture apochromatic objective. An interlaced acquisition approach is used to achieve simultaneous, co-registered imaging. The SD-OCT modality provides a 9.7 µm axial resolution. Comprehensive in vivo imaging of a nude mouse ear is demonstrated, with the SD-OCT scattering intensity revealing dermal morphology, and PARS microscopy providing a map of microvasculature.
我们开发了一种多模态成像平台，结合了光谱域光相干断层扫描（SD-OCT）的深度分辨散射对比，以及光声遥感（PARS）显微镜的互补非接触吸收对比。该系统使用远心扫描透镜提供宽场 OCT 模式，使用 0.26 数值孔径复消色差物镜提供高分辨率、双对比度模式。采用交错采集方法实现同时、配准成像。SD-OCT 模式提供 9.7 µm 的轴向分辨率。对裸鼠耳朵进行了全面的体内成像，SD-OCT 散射强度显示了真皮形态，而 PARS 显微镜则提供了微血管图谱。
