Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USA.
Exp Biol Med (Maywood). 2021 Apr;246(7):771-777. doi: 10.1177/1535370220977176. Epub 2020 Dec 9.
As a hybrid optical microscopic imaging technology, photoacoustic microscopy images the optical absorption contrasts and takes advantage of low acoustic scattering of biological tissues to achieve high-resolution anatomical and functional imaging. When combined with other imaging modalities, photoacoustic microscopy-based multimodal technologies can provide complementary contrast mechanisms to reveal complementary information of biological tissues. To achieve intrinsically and precisely registered images in a multimodal photoacoustic microscopy imaging system, either the ultrasonic transducer or the light source can be shared among the different imaging modalities. These technologies are the major focus of this minireview. It also covered the progress of the recently developed penta-modal photoacoustic microscopy imaging system featuring a novel dynamic focusing technique enabled by OCT contour scan.
作为一种混合光学显微成像技术，光声显微镜对光吸收对比度进行成像，并利用生物组织的低声散射来实现高分辨率的解剖学和功能成像。当与其他成像模式结合使用时，基于光声显微镜的多模态技术可以提供互补的对比机制，以揭示生物组织的互补信息。为了在多模态光声显微镜成像系统中实现固有且精确的配准图像，可以在不同的成像模式之间共享超声换能器或光源。这些技术是本综述的主要重点。它还涵盖了最近开发的五模态光声显微镜成像系统的进展，该系统具有通过 OCT 轮廓扫描实现的新型动态聚焦技术。
