吲哚菁绿标记的早期结肠癌肝转移的原位裸鼠模型。
Indocyanine Green Labels an Orthotopic Nude-Mouse Model of Very-Early Colon-Cancer Liver Metastases.
机构信息
AntiCancer Inc, San Diego, CA, U.S.A.
Department of Surgery, University of California, San Diego, CA, U.S.A.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2277-2280. doi: 10.21873/invivo.12038.
BACKGROUND/AIM: Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) is used to visualize colon-cancer liver metastases (CCLM) during surgery. The present study aimed to use ICG to visualize small CCLM in an orthotopic mouse model.
MATERIALS AND METHODS
A 3-mm fragment of HT29 human colon cancer was implanted to the liver of 5 athymic nude mice (nu/nu). The Pearl Trilogy Small Animal Fluorescence Imaging system was used 24 h after intravenous (IV) injection of 0.025 mg (0.25 mg/ml) ICG.
RESULTS
In four of the five mice, tumor fluorescence was detected. Small tumors (approximately 3 mm) were distinctly visualized with a minimal fluorescence liver signal, with a mean tumor to liver signal ratio of 1.81 (SD±0.167).
CONCLUSION
Small CCLM can be clearly identified by ICG fluorescence in an orthotopic nude-mouse model. This model is translatable to the clinic for the detection of occult early CCLM.
背景/目的：近红外（NIR）荧光成像使用吲哚菁绿（ICG）用于在手术期间可视化结肠癌肝转移（CCLM）。本研究旨在使用 ICG 在原位小鼠模型中可视化小的 CCLM。
材料和方法
将 3mm 片段的 HT29 人结肠癌植入 5 只无胸腺裸鼠（nu/nu）的肝脏中。在静脉内（IV）注射 0.025mg（0.25mg/ml）ICG 后 24 小时使用 Pearl Trilogy 小动物荧光成像系统进行检测。
结果
在五只小鼠中的四只中，检测到肿瘤荧光。通过最小的肝脏荧光信号可以清楚地看到小肿瘤（约 3mm），肿瘤与肝脏信号的平均比值为 1.81（SD±0.167）。
结论
在原位裸鼠模型中，ICG 荧光可清晰识别小的 CCLM。该模型可转化为临床检测隐匿性早期 CCLM。
Zotero 插件