结扎法在原位裸鼠肝转移模型中特异性标记肝脏节段的吲哚菁绿。
Ligation Method to Specifically Label a Liver Segment With Indocyanine Green in an Orthotopic Nude-Mouse Liver-Metastasis Model.
机构信息
Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
AntiCancer, Inc., San Diego, CA, U.S.A.
出版信息
In Vivo. 2020 Nov-Dec;34(6):3159-3162. doi: 10.21873/invivo.12150.
BACKGROUND/AIM: The visualization of hepatic segments with indocyanine green (ICG) fluorescence can aid in anatomic liver resection. The present study aimed to develop a method to specifically label an hepatic segment in a nude mouse model with liver metastasis.
MATERIALS AND METHODS
An orthotopic mouse model was established by surgical orthotopic implantation (SOI) of a patient-derived colon-cancer liver metastasis in the left lobe of the liver. Three weeks after SOI, the left Glissonean pedicle was ligated and 10 μg ICG was administrated intravenously. Images were obtained with the Pearl Trilogy Imaging System.
RESULTS
All mice expressed an 800 nm signal from ICG on the right lobe of the liver. The left lobe of the liver, in which the tumor was located, showed no fluorescence and had ischemia due to successful ligation of the Glissonean pedicle.
CONCLUSION
The ligation of the Glissonean pedicle enables specific liver-segment labeling with ICG, which has potential clinical application for liver metastasectomy.
背景/目的：吲哚菁绿（ICG）荧光可用于可视化肝段，有助于解剖性肝切除术。本研究旨在开发一种方法，以在肝转移的裸鼠模型中特异性标记肝段。
材料和方法
通过手术原位植入（SOI）患者来源的结肠癌肝转移到左叶肝脏建立原位鼠模型。SOI 后 3 周，结扎左 Glissonean 蒂并静脉内给予 10μg ICG。使用 Pearl Trilogy 成像系统获取图像。
结果
所有小鼠的右叶肝脏均表达 ICG 的 800nm 信号。由于 Glissonean 蒂结扎成功，肿瘤所在的左叶肝脏没有荧光且发生缺血。
结论
Glissonean 蒂结扎可实现 ICG 特异性肝段标记，对肝转移切除术具有潜在的临床应用价值。
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