一种使用热可逆凝胶聚合物进行肝细胞低温保存的改良包封方法，用于功能性移植。
An Improved Encapsulation Method for Cryopreserving Hepatocytes for Functional Transplantation Using a Thermo-reversible Gelation Polymer.
机构信息
Department of Gastroenterological and General Surgery, School of Medicine, Showa University, Tokyo, Japan.
Department of Gastroenterological and General Surgery, School of Medicine, Showa University, Tokyo, Japan
出版信息
In Vivo. 2020 Sep-Oct;34(5):2309-2316. doi: 10.21873/invivo.12043.
BACKGROUND/AIM: Thermo-reversible gelation polymer (TGP) can be converted into a gel state upon warming and liquid upon cooling. The present study aimed to demonstrate a new method for cryopreservation and encapsulation of rat hepatocytes using a TGP and their successful transplantation.
MATERIALS AND METHODS
The isolated rat hepatocytes were microencapsulated using TGP, and stored in liquid nitrogen. After cryopreservation, hepatocytes were cultured. Moreover, hepatocytes were transplanted into the spleen without a TGP capsule.
RESULTS
The viability of hepatocytes that were cryopreserved in TGP was 71.2±2.3%. The hepatocytes demonstrated adequate survival, maintained their hepatic function in culture, and expressed albumin after transplantation to the rat spleen.
CONCLUSION
We demonstrated a cryopreservation method of rat hepatocyte encapsulation using a TGP gel in the hydrogel state which subsequently allowed successful transplantation of unencapsulated hepatocytes in a sol state TGP gel at low temperature.
背景/目的：温敏凝胶聚合物（TGP）在升温时可转变为凝胶状态，降温时则为液体状态。本研究旨在展示一种使用 TGP 进行大鼠肝细胞冷冻保存和包封的新方法，并实现其成功移植。
材料与方法
使用 TGP 将分离的大鼠肝细胞微囊化，并在液氮中储存。冷冻保存后，对肝细胞进行培养。此外，将没有 TGP 胶囊的肝细胞移植到脾脏中。
结果
在 TGP 中冷冻保存的肝细胞的存活率为 71.2±2.3%。这些肝细胞在培养中具有良好的生存能力，保持了其肝功能，并在移植到大鼠脾脏后表达了白蛋白。
结论
我们展示了一种使用 TGP 水凝胶对大鼠肝细胞进行冷冻保存和包封的方法，随后可以在低温下以溶胶状态 TGP 凝胶成功移植未包封的肝细胞。
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