Mitry Ragai R, Hughes Robin D, Dhawan Anil
Institute of Liver Studies, Guy's, King's, and St. Thomas' School of Medicine, and King's College Hospital, Denmark Hill, SE5 9RS, London, UK.
Semin Cell Dev Biol. 2002 Dec;13(6):463-7. doi: 10.1016/s1084952102001350.
The quality of the hepatocytes used for clinical cell transplantation is very important, and depends to a large extent on the nature of the tissue used for isolation. The collagenase perfusion technique to isolate hepatocytes from animal livers has been further developed for isolation of human hepatocytes. As the donor organ pool is a scarce resource, marginal livers unsuitable for transplantation and segments from reduced grafts remain the main source of tissue for cell isolation. Use of livers from non-heart beating donors and foetal livers may further increase the tissue pool. With the limited supply of available tissue, improvements in the cryopreservation protocols are required to maintain cell viability on thawing and establish hepatocyte banks.
用于临床细胞移植的肝细胞质量非常重要，并且在很大程度上取决于用于分离的组织的性质。从动物肝脏中分离肝细胞的胶原酶灌注技术已进一步发展用于分离人肝细胞。由于供体器官库是一种稀缺资源，不适合移植的边缘肝脏和来自缩小移植物的肝段仍然是细胞分离的主要组织来源。使用非心脏跳动供体的肝脏和胎儿肝脏可能会进一步增加组织库。鉴于可用组织供应有限，需要改进冷冻保存方案以在解冻时维持细胞活力并建立肝细胞库。
