Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel.
Genomic Bioinformatics Laboratory, Department of Molecular Biology, Ariel University, Ariel 40700, Israel.
Genes (Basel). 2020 Aug 28;11(9):1016. doi: 10.3390/genes11091016.
The intracellular pathogenic bacteria belonging to the genus must cope with acidic stress as they penetrate the host via the gastrointestinal route, and again during the initial stages of intracellular infection. A transcription-level regulation has been proposed to explain this but the specific molecular mechanisms are yet to be determined. We recently reported a comparative transcriptomic analysis of the attenuated vaccine strain Rev.1 against the virulent strain 16M in cultures grown under either neutral or acidic conditions. Here, we re-analyze the RNA-seq data of 16M from our previous study and compare it to published transcriptomic data of this strain from both an in cellulo and an in vivo model. We identify 588 genes that are exclusively differentially expressed in 16M grown under acidic versus neutral pH conditions, including 286 upregulated genes and 302 downregulated genes that are not differentially expressed in either the in cellulo or the in vivo model. Of these, we highlight 13 key genes that are known to be associated with a bacterial response to acidic stress and, in our study, were highly upregulated under acidic conditions. These genes provide new molecular insights into the mechanisms underlying the acid-resistance of within its host.
细胞内致病菌必须应对酸性应激，因为它们通过胃肠道途径穿透宿主，并且在细胞内感染的初始阶段再次面临这种应激。已经提出了转录水平的调节来解释这一点，但具体的分子机制尚待确定。我们最近报道了在中性或酸性条件下培养时，减毒疫苗菌株 Rev.1 与毒力菌株 16M 的比较转录组分析。在这里，我们重新分析了我们之前研究中 16M 的 RNA-seq 数据，并将其与来自细胞内和体内模型的该菌株的已发表转录组数据进行比较。我们确定了 588 个在酸性条件下生长的 16M 中特异性差异表达的基因，包括 286 个上调基因和 302 个下调基因，这些基因在细胞内或体内模型中均无差异表达。其中，我们强调了 13 个已知与细菌对酸性应激反应相关的关键基因，在我们的研究中，这些基因在酸性条件下高度上调。这些基因为宿主内 耐酸性的机制提供了新的分子见解。
