Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX, United States.
Microb Pathog. 2011 Nov;51(5):338-44. doi: 10.1016/j.micpath.2011.07.006. Epub 2011 Jul 20.
Brucella spp. infect hosts primarily by adhering and penetrating mucosal surfaces; however the initial molecular phenomena of this host:pathogen interaction remain poorly understood. Using cDNA microarray analysis, we characterized the transcriptional profile of the intracellular pathogen Brucella melitensis at 4 h (adaptational period) and 12 h (replicative phase) following HeLa cells infection. The intracellular pathogen transcriptome was determined using initially enriched and then amplified B. melitensis RNA from total RNA of B. melitensis-infected HeLa cells. Analysis of microarray results identified 161 and 115 pathogen genes differentially expressed at 4 and 12 h p.i., respectively. In concordance with phenotypic studies, most of the genes expressed were involved in pathogen growth and metabolism, and were down-regulated at the earliest time point (78%), but up-regulated at 12 h p.i. (75%). Further characterization of specific genes identified in this study will elucidate biological processes and pathways to help understand how both host and Brucella interact during the early infectious process to the eventual benefit of the pathogen and to the detriment of the naïve host.
布鲁氏菌属主要通过黏附和穿透黏膜表面感染宿主; 然而，这种宿主与病原体相互作用的初始分子现象仍知之甚少。我们使用 cDNA 微阵列分析，在 HeLa 细胞感染后 4 小时（适应期）和 12 小时（复制期），对细胞内病原体布鲁氏菌 melitensis 的转录谱进行了描述。使用最初从布鲁氏菌 melitensis 感染的 HeLa 细胞的总 RNA 中富集和扩增的布鲁氏菌 melitensis RNA 来确定细胞内病原体转录组。微阵列结果分析鉴定了 161 个和 115 个病原体基因，分别在 4 和 12 h p.i. 时差异表达。与表型研究一致，大多数表达的基因与病原体的生长和代谢有关，在最早的时间点（78%）下调，但在 12 h p.i.（75%）上调。对本研究中鉴定的特定基因的进一步表征将阐明生物学过程和途径，以帮助理解宿主和布鲁氏菌在早期感染过程中如何相互作用，最终有利于病原体，而不利于幼稚的宿主。
