Rungrod Amlika, Makarasen Arthit, Patnin Suwicha, Techasakul Supanna, Somsunan Runglawan
Laboratory of Organic Synthesis, Chulabhorn Research Institute, Bangkok 10210, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Polymers (Basel). 2025 Aug 7;17(15):2157. doi: 10.3390/polym17152157.
Developing a rapidly gel-forming, in situ sprayable hydrogel with wound dressing functionality is essential for enhancing the wound healing process. In this study, a novel sprayable hydrogel-based wound dressing was developed by combining thermo- and pH- responsive polymers including Pluronic F127 (PF127) and -succinyl chitosan (NSC). NSC was prepared by modifying chitosan with succinic anhydride, as confirmed by Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The NSC synthesized using a succinic anhydride-to-chitosan molar ratio of 5:1 exhibited the highest degree of substitution, resulting in a water-soluble polymer effective over a broad pH range. The formulation process of the PF127:NSC sprayable hydrogel was optimized and evaluated based on its sol-gel phase transition behavior, clarity, gelation time, liquid and moisture management, stability, and cytotoxicity. These properties can be suitably tailored by adjusting the concentrations of PF127 and NSC. Moreover, the antioxidant capacity of the hydrogels was enhanced by incorporating (neem) extract, a bioactive compound, into the optimized sprayable hydrogel. Both neem release and antioxidant activity increased in a dose-dependent manner. Overall, the developed sprayable hydrogel exhibited favorable sprayability, appropriate gelation properties, controlled drug release, and antioxidant activity, underscoring its promising translational potential as a wound dressing.
开发一种具有伤口敷料功能的快速凝胶形成的原位可喷涂水凝胶对于促进伤口愈合过程至关重要。在本研究中，通过将包括泊洛沙姆F127（PF127）和N-琥珀酰壳聚糖（NSC）在内的热响应和pH响应聚合物相结合，开发了一种新型的基于可喷涂水凝胶的伤口敷料。NSC是通过用琥珀酸酐修饰壳聚糖制备的，这通过傅里叶变换红外光谱和核磁共振光谱得到证实。使用琥珀酸酐与壳聚糖摩尔比为5:1合成的NSC表现出最高的取代度，从而得到一种在宽pH范围内有效的水溶性聚合物。基于其溶胶-凝胶相变行为、透明度、凝胶化时间、液体和水分管理、稳定性和细胞毒性，对PF127:NSC可喷涂水凝胶的配方工艺进行了优化和评估。通过调整PF127和NSC的浓度，可以适当地调整这些性能。此外，通过将生物活性化合物印楝提取物加入到优化的可喷涂水凝胶中，提高了水凝胶的抗氧化能力。印楝释放和抗氧化活性均呈剂量依赖性增加。总体而言，所开发的可喷涂水凝胶表现出良好的可喷涂性、合适的凝胶化性能、可控的药物释放和抗氧化活性，突出了其作为伤口敷料的有前景的转化潜力。
