Ichikawa Kana, Tanaka Sai-Ichi, Miyajima Masayasu, Okada Yuka, Saika Shizuya
Department of Ophthalmology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama, 641-0012, Japan.
Taiwan J Ophthalmol. 2020 Apr 10;10(2):100-105. doi: 10.4103/tjo.tjo_80_19. eCollection 2020 Apr-Jun.
We investigated the effect of systemic fasudil hydrochloride and an inhibitor of nuclear translocation of myocardin-related transcription factor-A (MRTF-A) on capsular contraction in a puncture-injured lens in mice.
Lens injury of an anterior capsular break was achieved in male adult C57Bl/6 mice under general and topical anesthesia at 1 h after systemic fasudil hydrochloride (intraperitoneal, 10 mg/kg body weight) or vehicle administration. The mice were allowed to heal after instillation of ofloxacin ointment, for 5 and 10 days with daily administration of fasudil hydrochloride or vehicle. In another series of experiment, we examined the effect of systemic administration of an MRTF-A inhibitor (CCG-203971, 100 mg/kg twice a day) on fibrogenic reaction and tissue contraction in an injured lens on day 5 or 10. The eye was processed for histology and immunohistochemistry for SM22, proliferating cell nuclear antigen (PCNA), or MRTF-A. In hematoxylin and eosin - stained samples, the distance between each edge of the break of the anterior capsule was measured and statistically analyzed.
A cluster of lens cell accumulation was formed adjacent to the edge of the capsular break on day 5. It contained cells labeled for SM22 and PCNA. The size of the cell cluster was larger in fasudil group of mice than in control mice on day 5. Systemic fasudil or CCG-203971 suppressed an excess contraction of the capsular break at certain time points.
Systemic administration of fasudil hydrochloride could be a treatment strategy of postoperative capsular contraction following cataract-intraocular lens surgery.
我们研究了全身性盐酸法舒地尔以及一种与心肌素相关转录因子-A（MRTF-A）核转位抑制剂对小鼠穿刺损伤晶状体囊膜收缩的影响。
在全身和局部麻醉下，于成年雄性C57Bl/6小鼠中造成前囊膜破裂的晶状体损伤，在全身性给予盐酸法舒地尔（腹腔注射，10毫克/千克体重）或赋形剂1小时后进行。滴注氧氟沙星眼膏后让小鼠愈合，每日给予盐酸法舒地尔或赋形剂，持续5天和10天。在另一系列实验中，我们在第5天或第10天研究了全身性给予MRTF-A抑制剂（CCG-203971，100毫克/千克，每日两次）对损伤晶状体纤维生成反应和组织收缩的影响。对眼睛进行组织学处理以及针对平滑肌22（SM22）、增殖细胞核抗原（PCNA）或MRTF-A的免疫组织化学处理。在苏木精和伊红染色的样本中，测量并统计分析前囊膜破裂各边缘之间的距离。
在第5天，在囊膜破裂边缘附近形成了一簇晶状体细胞聚集物。其中含有标记有SM22和PCNA的细胞。在第5天，法舒地尔组小鼠的细胞簇大小比对照小鼠的大。全身性给予法舒地尔或CCG-203971在某些时间点抑制了囊膜破裂的过度收缩。
全身性给予盐酸法舒地尔可能是白内障人工晶状体手术后囊膜收缩的一种治疗策略。
