循环外泌体微小RNA作为系统性红斑狼疮的生物标志物
Circulating Exosomal microRNAs as Biomarkers of Systemic Lupus Erythematosus.
作者信息
Li Wengen, Liu Sudong, Chen Yongyu, Weng Ruiqiang, Zhang Ke, He Xuechun, He Chunmei
机构信息
Rheumatology Department, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, P. R. China.
Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, P. R. China.
出版信息
Clinics (Sao Paulo). 2020;75:e1528. doi: 10.6061/clinics/2020/e1528. Epub 2020 Aug 26.
OBJECTIVES
Many studies indicate that microRNAs (miRNAs) could be potential biomarkers for various diseases. The purpose of this study was to investigate the clinical value of serum exosomal miRNAs in systemic lupus erythematosus (SLE).
METHODS
Serum exosomes were isolated from 38 patients with SLE and 18 healthy controls (HCs). The expression of miR-21, miR-146a and miR-155 within exosomes was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Using receiver operating characteristic (ROC) curves, we evaluated the diagnostic value of exosomal miRNAs.
RESULTS
Exosomal miR-21 and miR-155 were upregulated (p<0.01), whereas miR-146a expression (p<0.05) was downregulated in patients with SLE, compared to that in HCs. The expression of miR-21 (p<0.01) and miR-155 (p<0.05) was higher in SLE patients with lupus nephritis (LN) than in those without LN (non-LN). The analysis of ROC curves revealed that the expression of miR-21 and miR-155 showed a potential diagnostic value for LN. Furthermore, miR-21 (R=0.44, p<0.05) and miR-155 (R=0.33, p<0.05) were positively correlated with proteinuria. The expression of miR-21 was negatively associated with anti-SSA/Ro antibodies (R=-0.38, p<0.05), and that of miR-146a was negatively associated with anti-dsDNA antibodies (R=-0.39, p<0.05).
CONCLUSIONS
These findings suggested that exosomal miR-21 and miR-155 expression levels may serve as potential biomarkers for the diagnosis of SLE and LN.
目的
许多研究表明，微小RNA（miRNA）可能是各种疾病的潜在生物标志物。本研究旨在探讨血清外泌体miRNA在系统性红斑狼疮（SLE）中的临床价值。
方法
从38例SLE患者和18名健康对照（HC）中分离血清外泌体。通过逆转录定量聚合酶链反应（RT-qPCR）检测外泌体内miR-21、miR-146a和miR-155的表达。使用受试者工作特征（ROC）曲线，我们评估了外泌体miRNA的诊断价值。
结果
与HC相比，SLE患者外泌体miR-21和miR-155上调（p<0.01），而miR-146a表达下调（p<0.05）。狼疮性肾炎（LN）的SLE患者中miR-21（p<0.01）和miR-155（p<0.05）的表达高于无LN（非LN）的患者。ROC曲线分析显示，miR-21和miR-155的表达对LN具有潜在诊断价值。此外，miR-21（R=0.44，p<0.05）和miR-155（R=0.33，p<0.05）与蛋白尿呈正相关。miR-21的表达与抗SSA/Ro抗体呈负相关（R=-0.38，p<0.05），miR-146a的表达与抗双链DNA抗体呈负相关（R=-0.39，p<0.05）。
结论
这些发现表明，外泌体miR-21和miR-155的表达水平可能作为SLE和LN诊断中的潜在生物标志物。
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