外周血单个核细胞中miR-21和miR-155的表达升高作为狼疮性肾炎的潜在生物标志物。
Elevated expression of miR-21 and miR-155 in peripheral blood mononuclear cells as potential biomarkers for lupus nephritis.
作者信息
Khoshmirsafa Majid, Kianmehr Nahid, Falak Reza, Mowla Seyed Javad, Seif Farhad, Mirzaei Behnaz, Valizadeh Mohadeseh, Shekarabi Mehdi
机构信息
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Rheumatology, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Int J Rheum Dis. 2019 Mar;22(3):458-467. doi: 10.1111/1756-185X.13410. Epub 2018 Nov 5.
AIM
Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE). There is a great interest in using microRNAs (miRNAs) as diagnostic and prognostic biomarkers in autoimmune diseases.
MATERIALS AND METHODS
This study evaluated miR-16, miR-21, miR-141, miR-146a, and miR-155 expression levels in peripheral blood mononuclear cells (PBMCs) of 55 female SLE patients with absent, inactive, or active nephritis, and 30 healthy controls (HCs) using quantitative polymerase chain reaction.
RESULTS
MiR-21 and miR-155 levels were significantly greater in the active nephritis group than in the absent, inactive or HC groups. Moreover, receiver operating characteristic and logistic regression analyses revealed miR-21 and miR-155 were significant risk factors for LN.
CONCLUSION
Overexpression of miR-21 and miR-155 in PBMCs may participate in LN pathophysiology and these miRNAs could be used as biomarkers for the condition.
目的
狼疮性肾炎（LN）是系统性红斑狼疮（SLE）的一种严重并发症。人们对将微小RNA（miRNA）用作自身免疫性疾病的诊断和预后生物标志物有着浓厚兴趣。
材料与方法
本研究采用定量聚合酶链反应评估了55例无肾炎、肾炎不活动或活动的女性SLE患者以及30例健康对照（HC）外周血单个核细胞（PBMC）中miR-16、miR-21、miR-141、miR-146a和miR-155的表达水平。
结果
活动期肾炎组的miR-21和miR-155水平显著高于无肾炎组、肾炎不活动组或健康对照组。此外，受试者工作特征曲线和逻辑回归分析显示，miR-21和miR-155是LN的显著危险因素。
结论
PBMC中miR-21和miR-155的过表达可能参与LN的病理生理过程，这些miRNA可作为该疾病的生物标志物。
Zotero 插件