Escuela de Bioanálisis, Facultad de Ciencias de la Salud, Universidad de Carabobo, Aragua, Venezuela.
Laboratorio de Bacteriología, Hospital de los Samanes, Aragua, Venezuela.
Rev Peru Med Exp Salud Publica. 2020 Apr-Jun;37(2):239-245. doi: 10.17843/rpmesp.2020.372.4652. Epub 2020 Aug 28.
Typify the SCCmec cassette in methicillin-resistant strains of Staphylococcus aureus in clinical isolates from health centers in the State of Aragua-Venezuela and compare the presence of SCCmec genotypes among the state health centers and according to the type of infection.
81 MRSA strains from four health centers of the Aragua-Venezuela State were studied. Methicillin resistance was performed with the Kirby-Bauer method with oxacillin (1 µg) and cefoxitin (30 µg) disks. The mecA gene and SCCmec were analyzed by the multiple PCR technique.
Only 55 isolates (67.9%) amplified the mecA gene, and 24 strains (43.6%) amplified SCCmec. SCCmec type I was the most frequency, followed by SCCmec IV and SCCmec III, representing 62.5%, 25% and 12.5%, respectively. SCCmec I was predominant in health center A (80%), while in B and C 60% and 100% respectively were SCCmec IV. At health center D, 50% turned out to be SCCmec I and 50% SCCmec IVd. A relationship was found between the SCCmec and the health center with statistical significance. SCCmec I predominated in skin and soft tissue and respiratory infections with 63.2% and 50%, respectively. There was no association between genotype and type of infection with a p value greater than 0.05.
The prevalence of SCCmec I and IV will allow establishing new measures in the use of antibiotics and epidemiological control.
对委内瑞拉阿格拉瓦州卫生中心临床分离的耐甲氧西林金黄色葡萄球菌（MRSA）菌株中的 SCCmec 盒进行分型，并比较州内各卫生中心以及根据感染类型之间 SCCmec 基因型的存在情况。
研究了来自委内瑞拉阿格拉瓦州四个卫生中心的 81 株 MRSA 菌株。采用 Kirby-Bauer 法，用 oxacillin（1µg）和 cefoxitin（30µg）纸片进行甲氧西林耐药性检测。采用多重 PCR 技术分析 mecA 基因和 SCCmec。
仅 55 株（67.9%）扩增 mecA 基因，24 株（43.6%）扩增 SCCmec。SCCmec 类型 I 最为常见，其次是 SCCmec IV 和 SCCmec III，分别占 62.5%、25%和 12.5%。SCCmec I 在卫生中心 A 中占主导地位（80%），而在 B 和 C 中分别占 60%和 100%为 SCCmec IV。在卫生中心 D，50%为 SCCmec I，50%为 SCCmec IVd。SCCmec 与卫生中心之间存在关联，具有统计学意义。SCCmec I 在皮肤和软组织以及呼吸道感染中占主导地位，分别为 63.2%和 50%。基因型与感染类型之间没有关联，p 值大于 0.05。
SCCmec I 和 IV 的流行将为抗生素的使用和流行病学控制制定新的措施。
