TRIO Fertility Partners, Division of Reproductive Sciences, University of Toronto, 655 Bay St 11th floor, Toronto, ON, M5G 2K4, Canada.
Division of Reproductive Sciences, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Canada.
Reprod Sci. 2021 Mar;28(3):904-908. doi: 10.1007/s43032-020-00306-3. Epub 2020 Sep 2.
With the recent increased utilization of oocyte vitrification for the purpose of fertility preservation, information regarding the future fertility potential of the frozen oocytes is mandatory. Nowadays, there is a relative lack of data about prediction of assisted reproductive technique (ART) success relying on the retrieved oocytes. In the present study, we therefore aimed to investigate whether oocyte diameter might predict the quality of the developing embryo. A retrospective, single-center cohort study. Oocytes retrieved following controlled ovarian hyperstimulation cycles during 2016 and incubated in a time-lapse incubator system were analyzed. Oocytes were grouped by mean oocyte diameter (MOD) and incubated for 5 days before the final morphological evaluation done by an expert embryologist. A total of 471 cycles which yielded 3355 metaphase II oocytes were included in the analysis. Embryos developed from oocytes with MOD close to the average (Average 1SD < MOD < Average + 1SD) had increased good-quality blastulation rates compared with embryos that developed from very small or very large oocytes. Oocytes with MOD between 105.96 and 118.69 μm have better probability of becoming top-quality D5 blastocysts (17.1-17.4% grade 1 embryos). There is a correlation between oocyte's MOD and the embryo quality at day 5. The oocytes with near average MOD have a better chance to develop to a good-quality embryo. Therefore, the study suggests that MOD might serve as a predictor for embryo grading at day 5.
随着卵母细胞玻璃化冷冻技术在生育力保存中的广泛应用，有关冷冻卵母细胞未来生育潜能的信息是必不可少的。目前，关于依靠获取的卵母细胞预测辅助生殖技术（ART）成功率的相关数据相对较少。因此，本研究旨在探讨卵母细胞直径是否可以预测胚胎质量。这是一项回顾性、单中心队列研究。分析了 2016 年控制性卵巢超刺激周期中获取的卵母细胞，并在时差培养箱系统中孵育。根据平均卵母细胞直径（MOD）对卵母细胞进行分组，并在最终由专家胚胎学家进行形态学评估前孵育 5 天。共纳入 471 个周期，共获得 3355 枚 MII 期卵母细胞。与来自非常小或非常大卵母细胞的胚胎相比，来自 MOD 接近平均值（平均 1SD<MOD<平均+1SD）的卵母细胞发育的优质囊胚率增加。MOD 在 105.96μm 到 118.69μm 之间的卵母细胞更有可能成为优质 D5 囊胚（17.1%-17.4%的 1 级胚胎）。卵母细胞 MOD 与第 5 天胚胎质量之间存在相关性。MOD 接近平均值的卵母细胞有更好的机会发育成优质胚胎。因此，该研究表明，MOD 可能可作为第 5 天胚胎分级的预测指标。
