1Department of Pharmaceutical Microbiology, Gülhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey.
2Institute of Health Sciences, Kırıkkale University, Kırıkkale, Turkey.
Acta Vet Hung. 2020 Sep 2;68(2):117-122. doi: 10.1556/004.2020.00024.
Vancomycin-resistant enterococcus (VRE) is a global threat to public health. Knowledge about the occurrence of vanA-carrying enterococci in broiler and environmental samples is important as antibiotic resistance can be transferred to human bacteria. The aim of this study was to investigate the presence of VRE in broiler cloacal and environmental (house) samples and to genotype the isolates. In this study, 350 swabs were collected from broiler farms. All samples were plated onto enterococcus selective agar containing 6 mg/L vancomycin and 64 mg/L ceftazidime. Minimum inhibitory concentration (MIC) values were determined for vancomycin and teicoplanin. Vancomycin-resistant Enterococcus faecium (VREfm) was isolated from 6 out of 300 (2%) broiler cloacal samples and 13 out of 50 (26%) house samples. All E. faecium isolates had vanA genes. All VREfm isolates (19 isolates) were confirmed to be 95% similar to each other. In conclusion, although 20 years have passed since the ban on avoparcin in Turkey, the present study shows that VREfm isolates are still present in broiler production and especially in broiler houses, and most importantly, a major VREfm clone was isolated from broiler cloacal and house samples.
耐万古霉素肠球菌（VRE）是全球公共卫生的威胁。了解携带 vanA 基因的肠球菌在肉鸡和环境样本中的存在情况很重要，因为抗生素耐药性可能会转移到人类细菌中。本研究旨在调查肉鸡泄殖腔和环境（鸡舍）样本中 VRE 的存在情况，并对分离株进行基因分型。在这项研究中，从 350 个肉鸡养殖场采集了 350 个拭子。所有样本均接种于含有 6mg/L 万古霉素和 64mg/L 头孢他啶的肠球菌选择性琼脂平板上。测定了万古霉素和替考拉宁的最低抑菌浓度（MIC）值。从 300 个（2%）肉鸡泄殖腔样本和 50 个（26%）鸡舍样本中分离出 6 株和 13 株耐万古霉素屎肠球菌（VREfm）。所有屎肠球菌分离株均携带 vanA 基因。所有 VREfm 分离株（19 株）彼此之间相似度达到 95%。总之，尽管在土耳其禁止使用维吉尼亚霉素已经过去了 20 年，但本研究表明，VREfm 分离株仍存在于肉鸡生产中，尤其是在肉鸡鸡舍中，更重要的是，从肉鸡泄殖腔和鸡舍样本中分离出了主要的 VREfm 克隆。
