Tagesson C, Magnusson K E, Stendahl O
J Immunol. 1977 Aug;119(2):609-13.
When phagocytosis-resistant Salmonella typhimurium 395 MS bacteria sensitized with anti-MS IgG antibodies were incubated with liposomes composed of phosphatidylcholine, cholesterol, and dicetylphosphate, a previously sequestered liposomal marker (4-methylumbelliferylphosphate) was released. Unsensitized or F(ab')2-sensitized bacteria had no such effect. This perturbation was neither dependent upon negatively charged dicetylphosphate nor upon cholesterol. It was further evident that palmitoyl-poly(ethyleneglycol) caused release of the trapped marker in a similar way as sensitized bacteria. These findings demonstrate a similarity between sensitized bacteria and a hydrophobic probe and lend support to the hypothesis that the perturbation was brought about by hydrophobic interaction. The observations indicate that liposomes, like phagocytes, possess "receptor sites" for the activated part of IgG and raise the possibility that phagocytic effectors can operate in a relatively nonspecific manner.
当用抗MS IgG抗体致敏的抗吞噬作用的鼠伤寒沙门氏菌395 MS细菌与由磷脂酰胆碱、胆固醇和磷酸二鲸蜡酯组成的脂质体一起孵育时，一种先前被隔离的脂质体标记物（4-甲基伞形基磷酸酯）被释放出来。未致敏或F(ab')2致敏的细菌没有这种作用。这种扰动既不依赖于带负电荷的磷酸二鲸蜡酯，也不依赖于胆固醇。进一步明显的是，棕榈酰聚乙二醇以与致敏细菌类似的方式导致被困标记物的释放。这些发现证明了致敏细菌与疏水探针之间的相似性，并支持了扰动是由疏水相互作用引起的这一假设。这些观察结果表明，脂质体与吞噬细胞一样，对IgG的活化部分具有“受体位点”，并增加了吞噬效应物可以以相对非特异性方式发挥作用的可能性。
