Stjernström I, Magnusson K E, Stendahl O, Tagesson C
Infect Immun. 1977 Nov;18(2):261-5. doi: 10.1128/iai.18.2.261-265.1977.
Sensitization of smooth Salmonella typhimurium 395 MS bacteria with hyper-immune anti-MS immunoglobulin G (IgG) antibodies increased the liability to hydrophobic interaction as assessed by the affinity for a column of Octyl-Sepharose. After sensitization, the material originally eluted with 1 M (NH4)SO4 in a 0.01 M phosphate buffer (pH 6.8) was not desorbed until the ionic strength was reduced to nil, and 0.1% (vol/vol) Triton X-100 in the 0.01 M phosphate buffer was used as eluant. Furthermore, by including positively charged bis-trimethylamino-polyethylene glycol (PEG) or negatively charged bis-sulfoamino-PEG in an aqueous two-phase system of dextran T500 and PEG 6000, the partition of the IgG-sensitized bacteria was affected by either of the polymers, whereas that of the parent bacteria was not. The hydrophobic effect of IgG binding was enhanced by complement. With heat-inactivated complement, the effect of IgG was diminished. The F(ab')2 fragment showed a much lower capacity to promote a hydrophobic interaction than the complete IgG molecule.
用超免疫抗MS免疫球蛋白G（IgG）抗体致敏光滑型鼠伤寒沙门氏菌395 MS细菌，通过对辛基-琼脂糖柱的亲和力评估，增加了其对疏水相互作用的敏感性。致敏后，原本在0.01 M磷酸盐缓冲液（pH 6.8）中用1 M硫酸铵洗脱的物质，直到离子强度降至零才被解吸，并且在0.01 M磷酸盐缓冲液中使用0.1%（体积/体积）的 Triton X-100作为洗脱剂。此外，在葡聚糖T500和聚乙二醇6000的水两相系统中加入带正电荷的双三甲基氨基聚乙二醇（PEG）或带负电荷的双磺基氨基PEG，IgG致敏细菌的分配受这两种聚合物中任何一种的影响，而亲本细菌则不受影响。补体增强了IgG结合的疏水作用。对于热灭活的补体，IgG的作用减弱。F(ab')2片段促进疏水相互作用的能力比完整的IgG分子低得多。
