Department of Orthopedics, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, P.R. China.
Qiqihar Medical University, Qiqihar, P.R. China.
J Musculoskelet Neuronal Interact. 2020 Sep 1;20(3):398-403.
To explore the role of medial collateral ligament repair in knee osteoarthritis based on TLR4/ MyD88/ NF-κ inflammatory signaling pathway.
The modified Hulth method was used to establish models, which were divided into a repair group, a model group, and a sham operation group. The repair group was treated with medial ligament repair technology. Synovium and cartilage morphological changes were evaluated by hematoxylin-eosin staining to determine the degree of reparation. The cartilage was evaluated by the Mankin's score, and inflammatory factors in cartilage tissues were determined by ELISA. The changes in TLR4, MyD88, and NF-κB levels were analyzed using the real-time quantitative PCR and Western blot assays.
The synovial and cartilage damages in the repair group and the sham operation group were significantly alleviated compared to the model group. The Mankin's score of the model group was significantly lower than the other two groups. The expression of inflammatory factors in the repair group and the sham operation group were significantly lower than in the model group. The expressions of those factors in the repair group and the model group were higher than those in the model group.
Medial ligament repair can improve the cartilage morphology and delay the development and progression of knee osteoarthritis by inhibiting the TLR4/MyD88/NF-κB signaling pathway.
基于 TLR4/MyD88/NF-κ炎症信号通路探讨内侧副韧带修复在膝骨关节炎中的作用。
采用改良 Hulth 法建立模型，分为修复组、模型组和假手术组。修复组采用内侧韧带修复技术。苏木精-伊红（H&E）染色评估滑膜和软骨形态变化，确定修复程度。Mankin 评分评估软骨，ELISA 法测定软骨组织中炎症因子。实时定量 PCR 和 Western blot 法分析 TLR4、MyD88 和 NF-κB 水平变化。
与模型组相比，修复组和假手术组的滑膜和软骨损伤明显减轻。模型组的 Mankin 评分明显低于其他两组。修复组和假手术组的炎症因子表达明显低于模型组。修复组和模型组的表达均高于模型组。
内侧韧带修复可通过抑制 TLR4/MyD88/NF-κB 信号通路改善软骨形态，延缓膝骨关节炎的发生发展。
