Clinical Research Center, Institute of Physical Medicine and Rehabilitation, University of São Paulo Medical School, São Paulo, Brazil.
Division of Physical Medicine, Center for Acupuncture, Institute of Orthopedics and Traumatology, University of São Paulo, São Paulo, Brazil ; Department of Pathology, University of São Paulo, São Paulo, Brazil.
Clin Interv Aging. 2014 Jun 12;9:939-44. doi: 10.2147/CIA.S60330. eCollection 2014.
Fibromyalgia and osteoarthritis may present a relationship with the concentration of cytokines. The aim of this study was to compare the serum concentrations of IL-12p70, tumor necrosis factor, IL-10, IL-6, IL-1β, and IL-8 in patients with knee osteoarthritis and fibromyalgia.
The study included 53 women (71.2±7.6 years old) diagnosed with knee osteoarthritis with moderate-to-severe pain (visual analog scale >4) for at least 3 months. Sixty women (54.1±8.1 years old) diagnosed with fibromyalgia according to the American College of Rheumatology criteria and with moderate-to-severe pain (visual analog scale >4) also participated in this study. For the dosage of cytokines, blood was collected in the morning: 5 mL from the cubital vein. The material was centrifuged at 4°C, separated into 100 μL aliquots and stored at -80°C until processing. Serum concentrations of the studied cytokines were assessed using the BD Cytometric Bead Array method. Data were analyzed with Student's t-test and the Mann-Whitney U test.
We found higher levels of IL-6, IL-10, and IL-1β in fibromyalgia patients. After adjustment of age as a covariate, there was no statistically significant difference in the concentration of any cytokine between fibromyalgia and knee osteoarthritis patients.
Patients with knee osteoarthritis and fibromyalgia with the same duration and intensity of pain demonstrate similar concentrations of cytokines. Aging may play a role in cytokine profile, a finding not so extensively addressed in the literature and one that should be further investigated.
纤维肌痛症和骨关节炎可能与细胞因子浓度有关。本研究旨在比较膝骨关节炎和纤维肌痛症患者血清中白细胞介素 12p70、肿瘤坏死因子、白细胞介素 10、白细胞介素 6、白细胞介素 1β和白细胞介素 8 的浓度。
本研究纳入了 53 名女性（71.2±7.6 岁），这些女性被诊断为膝骨关节炎，且疼痛程度为中度至重度（视觉模拟评分>4）至少持续 3 个月。60 名女性（54.1±8.1 岁）被诊断为纤维肌痛症，这些女性符合美国风湿病学会标准，且疼痛程度为中度至重度（视觉模拟评分>4）。为了测定细胞因子的含量，于清晨从肘静脉采集 5mL 血液。将标本在 4°C 下离心，分离出 100μL 等分试样，并在-80°C 下储存直至处理。采用 BD 流式细胞术微珠阵列法测定所研究细胞因子的血清浓度。采用学生 t 检验和曼-惠特尼 U 检验对数据进行分析。
我们发现纤维肌痛症患者的白细胞介素 6、白细胞介素 10 和白细胞介素 1β 水平较高。在调整年龄为协变量后，纤维肌痛症和膝骨关节炎患者的细胞因子浓度无统计学差异。
疼痛持续时间和强度相同的膝骨关节炎和纤维肌痛症患者的细胞因子浓度相似。衰老可能在细胞因子谱中起作用，这在文献中尚未广泛涉及，需要进一步研究。
