Korean Peninsula Infrastructure Special Committee, Korea Institute of Civil Engineering and Building Technology, Goyang, South Korea.
School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea.
Biotechnol Bioeng. 2021 Jan;118(1):82-93. doi: 10.1002/bit.27552. Epub 2020 Sep 15.
Bacterial biofilm formation causes serious problems in various fields of medical, clinical, and industrial settings. Antibiotics and biocide treatments are typical methods used to remove bacterial biofilms, but biofilms are difficult to remove effectively from surfaces due to their increased resistance. An alternative approach to treatment with antimicrobial agents is using biofilm inhibitors that regulate biofilm development without inhibiting bacterial growth. In the present study, we found that linoleic acid (LA), a plant unsaturated fatty acid, inhibits biofilm formation under static and continuous conditions without inhibiting the growth of Pseudomonas aeruginosa. LA also influenced the bacterial motility, extracellular polymeric substance production, and biofilm dispersion by decreasing the intracellular cyclic diguanylate concentration through increased phosphodiesterase activity. Furthermore, quantitative gene expression analysis demonstrated that LA induced the expression of genes associated with diffusible signaling factor-mediated quorum sensing that can inhibit or induce the dispersion of P. aeruginosa biofilms. These results suggest that LA is functionally and structurally similar to a P. aeruginosa diffusible signaling factor (cis-2-decenoic acid) and, in turn, act as an agonist molecule in biofilm dispersion.
细菌生物膜的形成在医学、临床和工业等各个领域都造成了严重的问题。抗生素和杀生剂处理是用于去除细菌生物膜的典型方法，但由于其增加的抗性，生物膜很难从表面有效去除。一种替代的抗菌剂治疗方法是使用生物膜抑制剂，它可以在不抑制细菌生长的情况下调节生物膜的发育。在本研究中，我们发现植物不饱和脂肪酸亚油酸（LA）在静态和连续条件下抑制生物膜形成，而不抑制铜绿假单胞菌的生长。LA 还通过增加磷酸二酯酶活性降低细胞内环二鸟苷酸浓度，影响细菌的运动性、胞外聚合物的产生和生物膜的分散。此外，定量基因表达分析表明，LA 诱导与可扩散信号因子介导的群体感应相关的基因表达，可抑制或诱导铜绿假单胞菌生物膜的分散。这些结果表明，LA 在功能和结构上与铜绿假单胞菌的可扩散信号因子（顺-2-癸烯酸）相似，并且作为生物膜分散的激动剂分子。
