Progeneer Incorporation, 12, Digital-ro 31-gil, Guro-gu, Seoul, 08380, South Korea.
School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Suwon, Gyeonggi-do, 16419, South Korea.
Chembiochem. 2021 Jan 15;22(2):392-397. doi: 10.1002/cbic.202000477. Epub 2020 Oct 29.
With the advent of innovative genomic discovery toolkits such as RT-PCR, genetic information can be quickly decrypted, and this has resulted in significant progress in overcoming diseases. However, RT-PCR has the serious problem of frequent errors, and the demand for a new gene diagnostic system is emerging. Herein, we propose a universal coding system for the effective detection of short single-stranded DNA or RNA by using a topological transformation-based nano-barcoding technique (TNT). Our goal was to develop a dedicated diagnostic device that unifies the other gene groups, thus resulting in minimum testing. In a universal coding system consisting of two separate circulation structures, different gene groups become generalized into specific single genes with the same sequence by a strand-displacement reaction and are then amplified, eventually being quickly detected in one TNT system. Simple gene diagnostic systems like this make high-speed, point-of-care diagnostic technologies, and we are very confident that these will provide clinical gene detection in the near future.
随着 RT-PCR 等创新基因组发现工具包的出现，遗传信息可以快速解密，这为克服疾病带来了重大进展。然而，RT-PCR 存在频繁出错的严重问题，因此需要一种新的基因诊断系统。在这里，我们提出了一种通用的编码系统，通过拓扑转换纳米标记技术（TNT）有效地检测短的单链 DNA 或 RNA。我们的目标是开发一种专用的诊断设备，将其他基因群统一起来，从而实现最小的检测。在由两个单独的循环结构组成的通用编码系统中，通过链置换反应，不同的基因群被概括为具有相同序列的特定单基因，然后进行扩增，最终在一个 TNT 系统中快速检测到。像这样简单的基因诊断系统可以实现高速、即时的诊断技术，我们非常有信心，这些技术将在不久的将来为临床基因检测提供帮助。
