Babenko Iu S, Gnilomedova L E, Kukushkina N V
Antibiot Khimioter. 1988 Feb;33(2):115-20.
Dependence of lytic enzyme preparation activity on temperature and time of Staphylococcus incubation with the preparation was shown. A decrease in the activity with an increase in the ionic strength of the incubation solutions and protective effect of salts on the staphylococcal cells were observed. Possible combined use of the preparation with antibiotics was studied. The enzymatic preparation inactivated penicillins and cephalosporins at the account of the ability of lytic endopeptidases to hydrolyze the peptide bond of the beta-lactam ring. However, its combined use with many other antibiotics such as novobiocin, lincomycin, rifampicin, gramicidin, polymyxin, oleandomycin, streptomycin, kanamycin, tetracycline and levomycetin is quite possible.
研究表明，溶菌酶制剂的活性取决于与该制剂一起孵育的葡萄球菌的温度和时间。观察到随着孵育溶液离子强度的增加，活性降低，并且盐对葡萄球菌细胞具有保护作用。研究了该制剂与抗生素联合使用的可能性。由于溶菌性内肽酶能够水解β-内酰胺环的肽键，该酶制剂可使青霉素和头孢菌素失活。然而，它与许多其他抗生素如新生霉素、林可霉素、利福平、短杆菌肽、多粘菌素、竹桃霉素、链霉素、卡那霉素、四环素和氯霉素联合使用是完全可行的。
