Touzet N, Jeanmaire-Zylberberg R, Chaminade M
J Embryol Exp Morphol. 1977 Jun;39:1-7.
The adult neuro-specific antigens have been localized by immunofluorescence techniques in diencephalon and mesencephalon of chick embryo. This study has been made using fresh or fixed tissues from embryos 72, 48 or 36 h old. At 72 h of incubation the wall of diencephalon shows marked fluorescence; at 48 h of incubation the fluorescent cells are localized in an outer layer and an inner one. In the 48 h-old embryo the reaction is more distinct and intensive in fresh tissues than in fixed tissues. At 36 h of incubation no fluorescence has been detected either in fresh tissues or in fixed tissues.
利用免疫荧光技术已将成年神经特异性抗原定位在鸡胚的间脑和中脑。本研究使用了72、48或36小时龄胚胎的新鲜或固定组织。在孵化72小时时，间脑壁呈现明显的荧光；在孵化48小时时，荧光细胞位于外层和内层。在48小时龄的胚胎中，新鲜组织中的反应比固定组织中的更明显和强烈。在孵化36小时时，无论是新鲜组织还是固定组织中均未检测到荧光。
