[链霉菌菌株原生质体形成与转化的方法]

[The methods of protoplast formation and transformation of Streptomyces strains].

作者信息

Furs A R, Orekhov A V

出版信息

Antibiot Khimioter. 1988 Mar;33(3):180-5.

Abstract

Factors influencing formation, regeneration and transformation of protoplasts in streptomyces are described. Conditions for formation and regeneration of protoplasts in 4 industrial strains producing the macrolide antibiotic tylosin were studied. It was demonstrated possible to apply the method for transformation of the S. lividans type culture to 3 industrial strains of S. griseus producing grisin, an antibiotic used as a feed additive. Potential increasing of the efficiency of protoplast transformation and transfection in various actinomycetous strains including industrial ones is discussed. The stimulating effect of lyposomes on transformation of protoplasts in S. lividans 66 with DNA of plasmids pVG101 and pIJ350 as well as transfection with DNA of phages SH10 and KS404 was shown. The tylosin resistance genes in S. fradiae strain B45 were cloned which enabled isolating the cluster of the genes participating in tylosin biosynthesis.

摘要

描述了影响链霉菌原生质体形成、再生和转化的因素。研究了4种生产大环内酯类抗生素泰乐菌素的工业菌株中，原生质体形成和再生的条件。结果表明，可将产二素链霉菌模式菌株的转化方法应用于3种生产放线菌素（一种用作饲料添加剂的抗生素）的灰色链霉菌工业菌株。讨论了提高包括工业菌株在内的各种放线菌菌株原生质体转化和转染效率的可能性。结果显示，脂质体对变铅青链霉菌66原生质体用质粒pVG101和pIJ350的DNA进行转化以及用噬菌体SH10和KS404的DNA进行转染具有刺激作用。弗氏链霉菌菌株B45中的泰乐菌素抗性基因被克隆，这使得参与泰乐菌素生物合成的基因簇得以分离。