Hranueli D, Smokvina T, Alacević M
Microbiologica. 1986 Jul;9(3):387-92.
One of the basic techniques for DNA cloning in Streptomyces is the preparation of protoplasts and the efficient regeneration of normal mycelia. In order to develop an oxytetracycline producing S. rimosus strain as a host for molecular cloning, the efficiencies of protoplast preparation and cell wall regeneration were compared with those obtained using S. lividans, the host for the majority of cloning experiments. The results presented suggest that the S. rimosus strain selected is a convenient host for the cloning of recombinant DNA, at least in relation to the preparation and regeneration of protoplasts. Interestingly, the size of the protoplasts was dependent upon the physiological age of the mycelium in S. rimosus; no such dependence was observed in S. lividans.
链霉菌中DNA克隆的基本技术之一是原生质体的制备以及正常菌丝体的高效再生。为了培育出一种生产土霉素的龟裂链霉菌菌株作为分子克隆的宿主，将原生质体制备和细胞壁再生的效率与使用作为大多数克隆实验宿主的天蓝色链霉菌所获得的效率进行了比较。所呈现的结果表明，所选的龟裂链霉菌菌株至少在原生质体制备和再生方面是重组DNA克隆的便利宿主。有趣的是，龟裂链霉菌中原生质体的大小取决于菌丝体的生理年龄；而在天蓝色链霉菌中未观察到这种依赖性。
