Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico.
Dirección, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico.
J Invertebr Pathol. 2020 Oct;176:107457. doi: 10.1016/j.jip.2020.107457. Epub 2020 Sep 1.
The spiny lobster Panulirus argus (Latreille, 1804) is currently affected by an unenveloped, icosahedral, DNA virus termed Panulirus argus virus 1 (PaV1), a virulent and pathogenic virus that produces a long-lasting infection that alters the physiology and behaviour of heavily infected lobsters. Gut-associated microbiota is crucial for lobster homeostasis and well-being, but pathogens could change microbiota composition affecting its function. In PaV1 infection, the changes of gut-associated microbiota are yet to be elucidated. In the present study, we used high-throughput 16S rRNA sequencing technology to compare the bacterial microbiota in intestines of healthy and heavily PaV1-infected male and female juveniles of spiny lobsters P. argus captured in Puerto Morelos Reef lagoon, Quintana Roo, Mexico. We found that basal gut-associated microbiota composition showed a sex-dependent bias, with females being enriched in amplicon sequence variants (ASVs) assigned to Sphingomonas, while males were enriched in the genus Candidatus Hepatoplasma and Aliiroseovarius genera. Moreover, the alpha diversity of microbiota decreased in PaV1-infected lobsters. A significant increase of the genus Candidatus Bacilloplasma was observed in infected lobsters, as well as a significant decrease in Nesterenkonia, Caldalkalibacillus, Pseudomonas, Cetobacterium and Phyllobacterium. We also observed an alteration in the abundances of Vibrio species. Results from this study suggest that PaV1 infection impacts intestinal microbiota composition in Panulirus argus in a sex-dependent manner.
棘皮十足目龙虾 Panulirus argus（拉特雷尔，1804 年）目前受到一种无包膜的二十面体 DNA 病毒的影响，该病毒称为 Panulirus argus 病毒 1（PaV1），这是一种致命和致病性的病毒，会导致持久感染，改变受感染龙虾的生理和行为。肠道相关微生物群对龙虾的体内平衡和健康至关重要，但病原体可能会改变微生物群的组成，从而影响其功能。在 PaV1 感染中，肠道相关微生物群的变化尚未阐明。在本研究中，我们使用高通量 16S rRNA 测序技术比较了在墨西哥金塔纳罗奥州 Puerto Morelos 礁泻湖捕获的健康和严重感染 PaV1 的雄性和雌性幼棘皮十足目龙虾 P. argus 肠道中的细菌微生物群。我们发现，基础肠道相关微生物群组成存在性别依赖性偏倚，雌性丰富的扩增子序列变体（ASVs）被分配给鞘氨醇单胞菌，而雄性丰富的是候选属 Hepato 菌和 Aliiroseovarius 属。此外，PaV1 感染的龙虾中微生物群的 alpha 多样性减少。在感染的龙虾中观察到候选属 Bacilloplasma 的数量显著增加，同时 Nesterenkonia、Caldalkalibacillus、Pseudomonas、Cetobacterium 和 Phyllobacterium 的数量显著减少。我们还观察到 Vibrio 种的丰度发生改变。本研究结果表明，PaV1 感染以性别依赖的方式影响 Panulirus argus 的肠道微生物群组成。
