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不同体型中华绒螯蟹的肌肉质地、组织化学和蛋白质组成。

Meat texture, muscle histochemistry and protein composition of Eriocheir sinensis with different size traits.

机构信息

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, China.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

出版信息

Food Chem. 2021 Feb 15;338:127632. doi: 10.1016/j.foodchem.2020.127632. Epub 2020 Jul 25.

Abstract

The main aim of the present work was to investigate the effect of the inherent differences in the raw muscle on the textural quality of the cooked meat from different sized Eriocheir sinensis. The content of entrapped water was 73.8-77.7 g/100 g in raw muscle. The density and diameter of muscle thick microfilaments ranged between 137 and 158/μm and 20.9-27.0 nm. These results demonstrated that the raw muscle from the tender group had a smaller density of larger diameter thick microfilaments and more entrapped water than other samples, which could explain the high tenderness of the cooked meat (P < 0.05). The potential structural proteins that indicated tenderness include myosin regulatory light chain 2, ancient ubiquitous protein 1, tubulin α-2 chain, and β-catenin, was determined using liquid chromatography-tandem mass spectrometry. The inherent attributes of raw muscle could explain the textural differences of the cooked meat from Eriocheir sinensis.

摘要

本研究旨在探讨生肌肉的固有差异对不同规格中华绒螯蟹熟肉质地的影响。生肌肉中的结合水含量为 73.8-77.7 g/100 g。肌肉粗微丝的密度和直径在 137-158/μm 和 20.9-27.0 nm 之间。这些结果表明,嫩组生肌肉的粗微丝密度较小,直径较大,结合水较多,这可以解释其熟肉的高嫩度(P < 0.05)。采用液相色谱-串联质谱法测定了表明嫩度的潜在结构蛋白,包括肌球蛋白调节轻链 2、古老普遍蛋白 1、微管蛋白 α-2 链和β-连环蛋白。生肌肉的固有特性可以解释中华绒螯蟹熟肉质地的差异。

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