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利用气相色谱-质谱联用嗅觉测量法对中华绒螯蟹雄蟹蒸煮后重要气味物质的表征

Characterization of important odorants in steamed male Chinese mitten crab (Eriocheir sinensis) using gas chromatography-mass spectrometry-olfactometry.

作者信息

Wu Na, Gu Saiqi, Tao Ningping, Wang Xichang, Ji Siru

机构信息

College of Food Science and Technology, Shanghai Ocean Univ, Nr. 999, Hucheng Huan Rd, Lingang New City, Shanghai, 201306, China.

出版信息

J Food Sci. 2014 Jul;79(7):C1250-9. doi: 10.1111/1750-3841.12511. Epub 2014 Jun 24.

DOI:10.1111/1750-3841.12511
PMID:24962135
Abstract

Chinese mitten crab (Eriocheir sinensis) from Yangcheng Lake in Jiangsu Province is a popular species due to its unique pleasant aroma and intensive umami taste. In this study, odorants in steamed male E. sinensis were investigated using the headspace-monolithic material sorptive extraction technique coupled with gas chromatography-mass spectrometry-olfactometry (GC-MS-O). A total of 74 volatile compounds were found, and the results of the GC-MS-O analysis, combined with odor activity values, showed that trimethylamine (fishy, ammonia-like odor), (Z)-4-heptenal (mushroom-like odor), and benzaldehyde (paint-like odor) were the important odorants (IOs) in all 4 of the edible parts of steamed male E. sinensis. Furthermore, heptanal (mushroom-like odor) was common to the abdomen, claw, and leg meat but was not found as the IO in the gonad. The abdomen meat also contained 3-methylbutanal (vegetable-like, grassy odor), while 2 additional IOs were found in claw meat (2-methylbutanal, which has a mushroom odor and 3-ethyl-2,5-dimethylpyrazine, which has a chocolate-like, musty odor). Another IO (2-nonanone, chocolate-like odor) was also found in leg meat, while (E)-2-nonenal (green, fruity odor) was the IO found exclusively in the gonad.

摘要

来自江苏省阳澄湖的中华绒螯蟹因其独特宜人的香气和浓郁的鲜味而备受欢迎。在本研究中,采用顶空-整体材料吸附萃取技术结合气相色谱-质谱-嗅闻联用仪(GC-MS-O)对清蒸雄蟹中的气味物质进行了研究。共发现74种挥发性化合物,GC-MS-O分析结果结合气味活性值表明,三甲胺(鱼腥味、氨味)、(Z)-4-庚烯醛(蘑菇味)和苯甲醛(油漆味)是清蒸雄蟹所有4个可食用部位中的重要气味活性物质(IOs)。此外,庚醛(蘑菇味)在腹部、爪子和腿部肌肉中均有发现,但在性腺中未被发现为IO。腹部肌肉还含有3-甲基丁醛(蔬菜味、草味),而在爪子肌肉中发现了另外2种IO(有蘑菇气味的2-甲基丁醛和有巧克力味、霉味的3-乙基-2,5-二甲基吡嗪)。腿部肌肉中还发现了另一种IO(有巧克力味的2-壬酮),而(E)-2-壬烯醛(绿色、水果味)是仅在性腺中发现的IO。

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