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通过萎蔫和接种剂处理对意大利黑麦草(多花黑麦草)青贮发酵模式和品质的影响

Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments.

作者信息

Liu Chang, Zhao Guo Qiang, Wei Sheng Nan, Kim Hak Jin, Li Yan Fen, Kim Jong Geun

机构信息

Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea.

Research Institute of Eco-friendly Livestock Science, Institute of GreenBio Science Technology, Seoul National University, Pyeongchang 25354, Korea.

出版信息

Anim Biosci. 2021 Jan;34(1):48-55. doi: 10.5713/ajas.20.0336. Epub 2020 Aug 21.

Abstract

OBJECTIVE

This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage.

METHODS

Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 106 cfu/g of Lactobacillus plantarum. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns.

RESULTS

Wilting treatment resulted in lower crude protein and in vitro dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and in vitro dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage.

CONCLUSION

The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

摘要

目的

本研究旨在探讨萎蔫和微生物接种剂处理对意大利黑麦草青贮发酵模式和品质的影响。

方法

意大利黑麦草在抽穗期收获,装入乙烯袋(20厘米×30厘米)青贮60天。意大利黑麦草分为4种处理方式(NWNA,不萎蔫不添加;NWA,不萎蔫添加;WNA,萎蔫不添加;WA,萎蔫添加),重复3次,萎蔫时间为5小时,添加物为每克含106 cfu的植物乳杆菌。在青贮后1、2、3、5、10、20、30、45和60天采集青贮样品,分析青贮品质和发酵模式特征。

结果

萎蔫处理导致粗蛋白和体外干物质消化率降低,酸性洗涤纤维(ADF)、总可消化养分(TDN)、水溶性碳水化合物(WSC)、氨含量和pH值无显著差异(p>0.05)。然而,萎蔫处理使意大利黑麦草青贮的ADF和中性洗涤纤维含量升高(p<0.05),WNA处理的TDN和体外干物质消化率最低。青贮的pH值在萎蔫组(WNA和WA)较高,在添加处理组较低。同时,pH值在储存第3 - 5天急剧下降。添加处理的氨氮含量显著较低(p<0.05),萎蔫无影响。随着发酵进行,乳酸和乙酸含量增加,在储存30天时含量最高。

结论

萎蔫处理未显著改善青贮发酵,但接种剂处理改善了青贮的发酵模式和品质。因此,制备高质量意大利黑麦草青贮时,建议青贮前接种,萎蔫时建议结合接种以制作高质量青贮。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/7888503/483ea73574f8/ajas-20-0336f1.jpg

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