Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdansk, Poland.
Int J Mol Sci. 2020 Sep 1;21(17):6345. doi: 10.3390/ijms21176345.
A newly isolated bacteriophage infecting strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, has been classified as a member, according to electron microscopy characterization of the virions, composed of a 50 nm-diameter head and a long, flexible, noncontractable tail (219 × 12.5 nm). Analysis of the whole dsDNA genome of this phage showed that it consists of 40,197 bp and functional modules containing genes coding for proteins that are involved in DNA replication (including DNA polymerase/primase), morphogenesis, packaging and cell lysis. Mass spectrometry analysis allowed us to identify several phage-encoded proteins. vB_EfaS-271 reveals a relatively narrow host range, as it is able to infect only a few strains. On the other hand, it is a virulent phage (unable to lysogenize host cells), effectively and quickly destroying cultures of sensitive host bacteria, with a latent period as short as 8 min and burst size of approximately 70 phages per cell at 37 °C. This phage was also able to destroy biofilms formed by . These results contribute to our understanding of the biodiversity of bacteriophages, confirming the high variability among these viruses and indicating specific genetic and functional features of vB_EfaS-271.
一种新分离的感染 菌株的噬菌体已经被鉴定，包括确定其分子特征。该噬菌体命名为 vB_EfaS-271，根据病毒粒子的电子显微镜特征，属于 科，由一个 50nm 直径的头部和一个长而灵活、不可收缩的尾部（219×12.5nm）组成。该噬菌体全 dsDNA 基因组分析表明，它由 40197bp 组成，包含参与 DNA 复制（包括 DNA 聚合酶/引物酶）、形态发生、包装和细胞裂解的基因编码蛋白的功能模块。质谱分析使我们能够鉴定出几种噬菌体编码蛋白。vB_EfaS-271 显示出相对较窄的宿主范围，因为它只能感染少数 菌株。另一方面，它是一种毒性噬菌体（不能溶原化宿主细胞），能够有效地迅速破坏敏感宿主细菌的培养物，潜伏期短至 8 分钟，在 37°C 时每个细胞的爆发量约为 70 个噬菌体。该噬菌体还能够破坏 形成的生物膜。这些结果有助于我们了解噬菌体的生物多样性，证实了这些病毒之间的高度变异性，并表明了 vB_EfaS-271 的特定遗传和功能特征。
