Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdansk, Poland.
Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Int J Mol Sci. 2020 Nov 21;21(22):8821. doi: 10.3390/ijms21228821.
Two newly discovered bacteriophages, isolated from chicken feces and infecting strains, are described in this report. These phages have been named vB_Sen-TO17 and vB_Sen-E22, and we present their molecular and functional characterization. Both studied viruses are able to infect several strains and develop lytically, but their specific host ranges differ significantly. Electron microscopic analyses of virions have been performed, and full genome sequences were determined and characterized, along with molecular phylogenetic studies. Genomes of vB_Sen-TO17 (ds DNA of 41,658 bp) and vB_Sen-E22 (dsDNA of 108,987 bp) are devoid of homologs of any known or putative gene coding for toxins or any other proteins potentially deleterious for eukaryotic cells. Both phages adsorbed efficiently (>95% adsorbed virions) within 10 min at 42 °C (resembling chicken body temperature) on cells of most tested host strains. Kinetics of lytic development of vB_Sen-TO17 and vB_Sen-E22, determined in one-step growth experiments, indicated that development is complete within 30-40 min at 42 °C, whereas burst sizes vary from 9 to 79 progeny phages per cell for vB_Sen-TO17 and from 18 to 64 for vB_Sen-E22, depending on the host strain. Virions of both phages were relatively stable (from several percent to almost 100% survivability) under various conditions, including acidic and alkaline pH values (from 3 to 12), temperatures from -80 °C to 60 °C, 70% ethanol, chloroform, and 10% DMSO. These characteristics of vB_Sen-TO17 and vB_Sen-E22 indicate that these phages might be considered in further studies on phage therapy, particularly in attempts to eliminate from chicken intestine.
本文描述了从鸡粪便中分离到的两种新的噬菌体，它们可以感染 株。这些噬菌体被命名为 vB_Sen-TO17 和 vB_Sen-E22，我们对它们的分子和功能特性进行了描述。这两种研究病毒都能够感染几种 株并进行裂解，但它们的特定宿主范围有很大差异。我们对病毒粒子进行了电子显微镜分析，并确定和表征了全基因组序列，同时进行了分子系统发育研究。vB_Sen-TO17（41658 bp 的 dsDNA）和 vB_Sen-E22（108987 bp 的 dsDNA）的基因组不包含任何已知或假定的编码毒素或任何其他对真核细胞有害的蛋白的基因的同源物。两种噬菌体在 42°C（类似于鸡体温）下 10 分钟内高效吸附（超过 95%的吸附病毒粒子）在大多数测试宿主株的细胞上。一步生长实验中测定的 vB_Sen-TO17 和 vB_Sen-E22 的裂解发育动力学表明，在 42°C 下 30-40 分钟内发育完全，而爆发大小根据宿主株的不同，vB_Sen-TO17 为每个细胞 9-79 个噬菌体，vB_Sen-E22 为 18-64 个噬菌体。两种噬菌体的病毒粒子在各种条件下相对稳定（从几个百分点到几乎 100%的存活率），包括酸性和碱性 pH 值（从 3 到 12）、从-80°C 到 60°C 的温度、70%乙醇、氯仿和 10%DMSO。vB_Sen-TO17 和 vB_Sen-E22 的这些特性表明，这些噬菌体可以在噬菌体治疗的进一步研究中考虑，特别是在试图从鸡肠道中消除 的尝试中。
