Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Mar Genomics. 2020 Oct;53:100754. doi: 10.1016/j.margen.2020.100754. Epub 2020 Feb 28.
The marine bacterium Neptunomonas concharum was firstly characterized in 2012. It preferred to utilize acetate as the carbon source to accumulate poly-3-hydroxybutyrate (PHB) as intracellular carbon and energy storage. Here we report the genomic characteristics of N. concharum JCM17730. The complete genome sequence of N. concharum JCM17730 consists of 3,561,992 bp in one contig, without plasmid. Analysis of coding sequences revealed the presence of genomic features involved in acetate assimilation and PHB metabolism. The genome of N. concharum JCM17730 contains three genes encoding acetyl-CoA synthetase and two genes encoding isocitrate lyase. Three polyhydroxyalkanoate synthases and one polyhydroxyalkanoate depolymerase are scattered throughout the genomic DNA. The genome features provide interesting insights into the acetate and PHB metabolism of N. concharum JCM17730 and would facilitate further research on the genetic engineering of marine bacteria for efficient PHB production.
海洋细菌 Neptunomonas concharum 于 2012 年首次被描述。它喜欢利用乙酸作为碳源，积累聚-3-羟基丁酸酯（PHB）作为细胞内的碳和能量储存。在这里，我们报告了 N. concharum JCM17730 的基因组特征。N. concharum JCM17730 的完整基因组序列由一个连续体组成，大小为 3561992bp，没有质粒。对编码序列的分析揭示了参与乙酸同化和 PHB 代谢的基因组特征的存在。N. concharum JCM17730 的基因组包含三个编码乙酰辅酶 A 合成酶的基因和两个编码异柠檬酸裂解酶的基因。三个聚羟基烷酸合酶和一个聚羟基烷酸解聚酶散布在整个基因组 DNA 中。基因组特征为 N. concharum JCM17730 的乙酸和 PHB 代谢提供了有趣的见解，并将促进海洋细菌遗传工程高效生产 PHB 的进一步研究。
