The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1958-1961. doi: 10.1099/ijs.0.017756-0. Epub 2009 Oct 2.
A Gram-negative, motile, oxidase- and catalase-positive and facultatively aerobic bacterium, designated S3-22T, was isolated from marine sediment of the Nella Fjord, Antarctica. Strain S3-22T reduced nitrate to nitrite and grew at pH 6.0-8.0, at 4-25 degrees C and with 0.5-5% (w/v) NaCl. It contained Q-8 as the only respiratory quinone and summed feature 3 (C16:1omega7c and/or iso-C15:0 2-OH), C16:0 and C18:1omega7c as the major cellular fatty acids. The genomic DNA G+C content was 45.6 mol%. Phylogenetic analyses of 16S rRNA gene sequences showed that strain S3-22T was affiliated with the genus Neptunomonas, with 97.1% sequence similarity to Neptunomonas japonica JAMM 0745T and 94.8% to Neptunomonas naphthovorans NAG-2N-126T, the type strains of the only two recognized Neptunomonas species. DNA-DNA relatedness between strain S3-22T and N. japonica JCM 14595T was 20.4%. Strain S3-22T could be distinguished from the type strains of Neptunomonas species by several phenotypic properties. Based on the evidence from our polyphasic study, strain S3-22T represents a novel Neptunomonas species, for which the name Neptunomonas antarctica sp. nov. is proposed. The type strain is S3-22T (=CCTCC AB 209086T =KACC 14056T).
一株革兰氏阴性、运动性、氧化酶和接触酶阳性、兼性需氧细菌，被命名为 S3-22T，从南极洲内拉峡湾的海洋沉积物中分离得到。S3-22T 菌株可以将硝酸盐还原为亚硝酸盐，并在 pH6.0-8.0、4-25°C 和 0.5-5%（w/v）NaCl 的条件下生长。它含有 Q-8 作为唯一的呼吸醌，并且包含特征脂肪酸总和 3（C16:1ω7c 和/或 iso-C15:0 2-OH）、C16:0 和 C18:1ω7c。基因组 DNA G+C 含量为 45.6mol%。16S rRNA 基因序列的系统发育分析表明，S3-22T 菌株与 Neptunomonas 属密切相关，与 Neptunomonas japonica JAMM 0745T 的序列相似性为 97.1%，与 Neptunomonas naphthovorans NAG-2N-126T 的序列相似性为 94.8%，后两者是仅有的两个被认可的 Neptunomonas 种的模式株。S3-22T 菌株与 N. japonica JCM 14595T 之间的 DNA-DNA 同源性为 20.4%。S3-22T 菌株与 Neptunomonas 种的模式株在许多表型特性上可以区分开来。基于多相研究的证据，S3-22T 菌株代表了一个新的 Neptunomonas 种，建议将其命名为 Neptunomonas antarctica sp. nov.。其模式菌株为 S3-22T（=CCTCC AB 209086T=KACC 14056T）。
