Dulipsingh Latha, Ibrahim Danyal, Schaefer Ernst J, Crowell Rebecca, Diffenderfer Margaret R, Williams Kendra, Lima Colleen, McKenzie Jessica, Cook Lisa, Puff Jennifer, Onoroski Mary, Wakefield Dorothy B, Eadie Reginald J, Kleiboeker Steven B, Nabors Patricia, Hussain Syed A
Diabetes and Endocrinology Center, Saint Francis Hospital and Medical Center, Hartford, CT, United States.
Trinity Health Of New England, Hartford, CT, United States.
Transfus Apher Sci. 2020 Dec;59(6):102922. doi: 10.1016/j.transci.2020.102922. Epub 2020 Aug 25.
SARS-CoV-2 has infected millions worldwide. The virus is novel, and currently there is no approved treatment. Convalescent plasma may offer a treatment option. We evaluated trends of IgM/IgG antibodies/plasma viral load in donors and recipients of convalescent plasma. 114/139 (82 %) donors had positive IgG antibodies. 46/114 donors tested positive a second time by NP swab. Among those retested, the median IgG declined (p < 0.01) between tests. 25/139 donors with confirmed SARS-CoV-2 were negative for IgG antibodies. This suggests that having had the infection does not necessarily convey immunity, or there is a short duration of immunity associated with a decline in antibodies. Plasma viral load obtained on 35/39 plasma recipients showed 22 (62.9 %) had non-detectable levels on average 14.5 days from positive test versus 6.2 days in those with detectable levels (p < 0.01). There was a relationship between IgG and viral load. IgG was higher in those with non-detectable viral loads. There was no relationship between viral load and blood type (p = 0.87) or death (0.80). Recipients with detectable viral load had lower IgG levels; there was no relationship between viral load, blood type or death.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已在全球感染了数百万人。这种病毒是新型的，目前尚无获批的治疗方法。康复期血浆可能提供一种治疗选择。我们评估了康复期血浆供体和受体中IgM/IgG抗体/血浆病毒载量的变化趋势。114/139名（82%）供体的IgG抗体呈阳性。46/114名供体通过鼻咽拭子再次检测呈阳性。在那些再次检测的人中，两次检测之间IgG的中位数下降了（p<0.01）。139名确诊感染SARS-CoV-2的供体中有25名IgG抗体呈阴性。这表明感染并不一定能带来免疫力，或者存在与抗体下降相关的短期免疫期。对35/39名血浆受体进行的血浆病毒载量检测显示，22名（62.9%）在阳性检测后平均14.5天病毒载量不可检测，而病毒载量可检测的受体平均为6.2天（p<0.01）。IgG与病毒载量之间存在关联。病毒载量不可检测者的IgG水平较高。病毒载量与血型（p=0.87）或死亡（p=0.80）之间无关联。病毒载量可检测的受体IgG水平较低；病毒载量、血型与死亡之间无关联。
