Department of Microbiology, Gandhi Medical College and Hospital, Secunderabad, Telangana, India.
Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Telangana, India.
Indian J Med Microbiol. 2020 Apr-Jun;38(2):183-191. doi: 10.4103/ijmm.IJMM_20_151.
Infections with methicillin-resistant Staphylococcus aureus (MRSA) greatly influence clinical outcome. Molecular characterisation of MRSA can help to predict their spread and to institute treatment and hospital protocols.
The aim of this study is to understand the diversity of MRSA in a tertiary care hospital in Hyderabad, India.
Samples collected at Gandhi Medical College, Hyderabad, and designed to assess hospital-or community-associated MRSA (HA-MRSA or CA-MRSA).
MRSA were subjected to antibiotic susceptibility testing, pulsed-field gel electrophoresis (PFGE), spa typing, multi-locus sequence typing and staphylococcal cassette chromosome-mec (SCCmec) typing.
Discriminatory index and 95% confidence interval.
Of the 30 MRSA, (a) 18 and 12 were HA-MRSA and CA-MRSA, respectively, and (b) 23.3% and 6.6% displayed induced clindamycin and intermediate vancomycin resistance, respectively. Genetic diversity was evident from the presence of (a) 20 pulsotypes, (b) eight spa types, with the predominance of t064 (n = 9) and (c) seven sequence types (ST), with the preponderance of ST22 and ST8 (9 each). ST22 and ST8 were the most prevalent among HA-MRSA and CA-MRSA, respectively. SCCmec type IV was the most frequent (n = 8). 44.4% of HA-MRSA belonged to SCCmec IV and V, whereas 33.3% of CA-MRSA belonged to SCCmec I and III; 33.3% (5/15) of the isolates harbouring the pvl gene belonged to SCCmec IVC/H.
ST8 was a dominant type along with other previously reported types ST22, ST239, and ST772 from India. The observations highlight the prevalence of genetically diverse clonal populations of MRSA, suggesting potential multiple origins.
耐甲氧西林金黄色葡萄球菌（MRSA）感染极大地影响临床结果。对 MRSA 的分子特征分析有助于预测其传播，并制定治疗和医院方案。
本研究旨在了解印度海得拉巴甘地医学院三级护理医院的 MRSA 多样性。
在海得拉巴甘地医学院收集样本，旨在评估医院或社区相关的 MRSA（HA-MRSA 或 CA-MRSA）。
对 MRSA 进行抗生素敏感性测试、脉冲场凝胶电泳（PFGE）、spa 分型、多位点序列分型和葡萄球菌盒染色体 mec（SCCmec）分型。
鉴别指数和 95%置信区间。
30 株 MRSA 中，（a）18 株为 HA-MRSA，12 株为 CA-MRSA，（b）分别有 23.3%和 6.6%的菌株表现出诱导性克林霉素和中间万古霉素耐药性。存在（a）20 种脉冲型、（b）8 种 spa 型，其中 t064 占优势（n=9）和（c）7 种序列型（ST），其中 ST22 和 ST8 占优势（各 9 种），表明遗传多样性明显。ST22 和 ST8 分别是 HA-MRSA 和 CA-MRSA 中最常见的类型。SCCmec 型 IV 最为常见（n=8）。44.4%的 HA-MRSA 属于 SCCmec IV 和 V，而 33.3%的 CA-MRSA 属于 SCCmec I 和 III；携带 pvl 基因的 33.3%（5/15）株属于 SCCmec IVC/H。
ST8 是一种优势类型，还有其他以前在印度报道的类型 ST22、ST239 和 ST772。这些发现强调了 MRSA 具有遗传多样性的克隆群体的流行，表明存在潜在的多种起源。
