Department of Cellular and Molecular Biology, Faculty of Pharmacy, "Ovidius" University, Capitan Aviator Al. Serbanescu Street No. 6, Campus C, Constanta, Romania.
Department of General Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1, Bucharest, Romania.
Sci Rep. 2020 Sep 3;10(1):14550. doi: 10.1038/s41598-020-71348-5.
The present study evaluated the structural and ultrastructural characteristics of Rapana venosa egg capsules, starting from observations of their antifouling activity and mechanical resistance to water currents in mid-shore habitats. Optical microscopy, epifluorescence, and electron microscopy were used to evaluate the surface and structure of the R. venosa egg capsules. These measurements revealed an internal multilamellar structure of the capsule wall with in-plane distributions of layers with various orientations. It was found that the walls contained vacuolar structures in the median layer, which provided the particular characteristics. Mechanical, viscoelastic and swelling measurements were also carried out. This study revealed the presence and distribution of chitosan in the capsule of R. venosa. Chitosan identification in the egg capsule wall structure was carried out through SEM-EDX measurements, colorimetric assays, FT-IR spectra and physical-chemical tests. The biopolymer presence in the capsule walls may explain the properties of their surfaces as well as the mechanical resistance of the capsule and its resistance to chemical variations in the living environment.
本研究从贻贝卵囊的抗污活性和对中潮带生境水流的机械阻力开始，评估了其结构和超微结构特征。采用光学显微镜、荧光显微镜和电子显微镜来评估 R. venosa 卵囊的表面和结构。这些测量结果显示，胶囊壁具有内部的多层结构，层的平面分布具有各种取向。研究发现，壁的中层含有空泡结构，这提供了特殊的特征。还进行了机械、粘弹性和溶胀测量。这项研究揭示了 R. venosa 卵囊中壳聚糖的存在和分布。通过 SEM-EDX 测量、比色分析、FT-IR 光谱和物理化学测试，对卵囊壁结构中的壳聚糖进行了鉴定。生物聚合物在胶囊壁中的存在可以解释其表面的特性以及胶囊的机械阻力及其对生活环境中化学变化的抵抗力。
