Parada D David, Peña G Karla B, Vives Margarida, Molina Alicia, Mayayo Emilio, Riu Frances, Sabench Fàtima, Del Castillo Daniel
Pathology Service, University Hospital of Sant Joan. Faculty of Medicine, IISPV, "Rovira i Virgili" University, Reus, Tarragona, Spain.
Surgery Service, University Hospital of Sant Joan, Faculty of Medicine, IISPV, "Rovira i Virgili" University, Reus, Tarragona, Spain.
Diabetes Metab Syndr Obes. 2020 Aug 18;13:2855-2864. doi: 10.2147/DMSO.S260483. eCollection 2020.
The cellular distribution of ghrelin positive cells is not well defined. The aim of the study is to quantify and determine the distribution of ghrelin cells in gastric mucosa in patients with morbid obesity and relate this finding to gender, comorbidities, gastritis, and plasmatic levels of acyl and desacylghrelin before and after laparoscopic sleeve gastrectomy.
We performed a study on 61 patients with BMI≥50 kg/m operated by laparoscopic sleeve gastrectomy. Three gastric regions were analyzed by histopathology and immunohistology. Blood sample was taken before surgery, and at 6 and 12 months post-surgery, to study the plasma levels of ghrelin isoforms.
Ghrelin cells are present in all regions of the gastric mucosa, with a greater number in the body and the fundus. Difference was found in the antrum between male and female patients (p=0.018). Patients with arterial hypertension also showed a lower level of cells in antrum (p<0.05). Acylghrelin levels after surgery were significantly lower (32.83+5.6 pg/mL to 10.09+11.8 pg/mL, p<0.05). Values of desacylghrelin tended to decrease but no significant variation was observed (207.4+39.3 pg/mL to 188.84+52.3 pg/mL).
Our patients show ghrelin cells in all areas of the stomach. Gender, comorbidities, and gastritis are determinant on gastric ghrelin-producing cells distribution.
胃饥饿素阳性细胞的细胞分布尚不明确。本研究的目的是量化并确定病态肥胖患者胃黏膜中胃饥饿素细胞的分布，并将这一发现与性别、合并症、胃炎以及腹腔镜袖状胃切除术前和术后酰基胃饥饿素和去酰基胃饥饿素的血浆水平相关联。
我们对61例接受腹腔镜袖状胃切除术、BMI≥50 kg/m²的患者进行了研究。通过组织病理学和免疫组织学分析三个胃区域。在手术前、术后6个月和12个月采集血样，以研究胃饥饿素异构体的血浆水平。
胃饥饿素细胞存在于胃黏膜的所有区域，胃体和胃底的数量更多。在胃窦部发现男性和女性患者存在差异（p = 0.018）。患有动脉高血压的患者胃窦部的细胞水平也较低（p < 0.05）。术后酰基胃饥饿素水平显著降低（从32.83 + 5.6 pg/mL降至10.09 + 11.8 pg/mL，p < 0.05）。去酰基胃饥饿素的值趋于下降，但未观察到显著变化（从207.4 + 39.3 pg/mL降至188.84 + 52.3 pg/mL）。
我们的患者在胃的所有区域均显示有胃饥饿素细胞。性别、合并症和胃炎是胃中产生胃饥饿素细胞分布的决定因素。
