Shan Yifan, Guo Jing, Fan Wei, Li Huijun, Wu Hui, Song Yong, Jalleh Geoffrey, Wu Weidong, Zhang Guicheng
School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province, 4353003, P.R. China.
Henan International Laboratory for Air Pollution Health Effects and Intervention, Xinxiang, Henan Province, 4353003, P.R. China.
World Allergy Organ J. 2020 Aug 20;13(8):100452. doi: 10.1016/j.waojou.2020.100452. eCollection 2020 Aug.
The prevalence of allergy and other common chronic diseases is higher in developed than developing countries, and higher in urban than rural regions. Urbanization through its modification of environmental microbiomes may play a predominant role in the development of these conditions. However, no studies have been conducted to compare the microbiome in house dust among areas with different urbanization levels.
House dust from Xinxiang rural area (XR, n = 74), Xinxiang urban area (XU, n = 33), and Zhengzhou urban area (ZU, n = 32) in central China, and from Australia (AU, n = 58 [with pets AUP, n = 15, without pets AUNP, n = 43]) were collected during a summer season in China and Australia. High-throughput sequencing of 16S rDNA was employed to profile house dust bacterial communities.
Settled dust collected in China was dominant with 2 bacterial phyla: Proteobacteria and Actinobacteria, while floor dust collected in Australia had a higher proportion of phylum Proteobacteria, Firmicutes, and Actinobacteria. XR dust samples presented higher bacterial richness and diversity compared with XU or ZU samples. Urbanization level (r = 0.741 < 0.001) had a significant correlation with the distribution of house dust bacterial community. At the genus level, there was a positive correlation (r coefficient > 0.5) between urbanization level and bacterial genera and a negative correlation (r coefficient < -0.5) with .
There was a significant difference in house dust microbiota among different urbanization areas. The areas with a lower urbanization level presented higher dust-borne bacterial richness and diversity. Modern urbanization has a significant influence on the bacterial microbiome profiles of indoor dust.
过敏及其他常见慢性病在发达国家的患病率高于发展中国家，在城市地区高于农村地区。城市化通过改变环境微生物群可能在这些疾病的发生发展中起主要作用。然而，尚未有研究比较不同城市化水平地区室内灰尘中的微生物群。
在中国中部的新乡农村地区（XR，n = 74）、新乡市区（XU，n = 33）、郑州市区（ZU，n = 32）以及澳大利亚（AU，n = 58 [有宠物AUP，n = 15，无宠物AUNP，n = 43]），于中国和澳大利亚的夏季采集室内灰尘。采用16S rDNA高通量测序对室内灰尘细菌群落进行分析。
在中国采集的沉降灰尘中，以变形菌门和放线菌门这2个细菌门为主，而在澳大利亚采集的地板灰尘中，变形菌门、厚壁菌门和放线菌门的比例更高。与XU或ZU样本相比，XR灰尘样本呈现出更高的细菌丰富度和多样性。城市化水平（r = 0.741，P < 0.001）与室内灰尘细菌群落分布显著相关。在属水平上，城市化水平与细菌属之间呈正相关（r系数> 0.5），与……呈负相关（r系数< -0.5）。
不同城市化地区的室内灰尘微生物群存在显著差异。城市化水平较低的地区呈现出更高的灰尘携带细菌丰富度和多样性。现代城市化对室内灰尘的细菌微生物群谱有显著影响。
