Glazunov A V, Boreĭko A V, Esser A Kh
Genetika. 1988 Mar;24(3):428-35.
gamma-induced reciprocal mitotic recombination and gene conversion have been studied under conditions inhibiting "rapid" postirradiation recovery of diploid yeast Saccharomyces cerevisiae. It turned out that, if the first postirradiation cell division occurs at higher KCl concentrations ("rapid" postirradiation recovery is inhibited), the frequency of mitotic reciprocal recombination within the gene ADE2-centromere region decreases. Keeping of irradiated cells (in the G1 phase of the cell division cycle) in water at 28 degrees C prior to plating on the selective agar containing 1.5 M KCl leads to smaller frequency of gene conversion lys2-25/lys2-22----Lys+, as compared with that for the cells immediately plated on the selective agar. Correlation has been found between the coefficient of gene conversion frequency decrease, due to postirradiation keeping in water, and "rapid" recovery efficiency. Interpretation of the data is based on the hypothesis that recombination repair of DNA double-strand breaks induced by ionizing radiation is responsible for "rapid" postirradiation recovery.
在抑制二倍体酿酒酵母“快速”辐照后恢复的条件下，研究了γ诱导的相互有丝分裂重组和基因转换。结果表明，如果辐照后的第一次细胞分裂发生在较高的KCl浓度下（“快速”辐照后恢复受到抑制），基因ADE2 - 着丝粒区域内有丝分裂相互重组的频率会降低。与立即接种在含有1.5 M KCl的选择性琼脂上的细胞相比，在28℃的水中将辐照细胞（处于细胞分裂周期的G1期）保存至接种到选择性琼脂上，会导致lys2 - 25/lys2 - 22----Lys +基因转换的频率降低。已发现由于辐照后保存在水中导致的基因转换频率降低系数与“快速”恢复效率之间存在相关性。数据解释基于这样的假设，即电离辐射诱导的DNA双链断裂的重组修复是“快速”辐照后恢复的原因。
