Thagfan Felwa A, Al-Megrin Wafa A, Al-Shaebi Esam M, Al-Quraishy Saleh, Dkhil Mohamed A
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Comb Chem High Throughput Screen. 2021;24(10):1603-1608. doi: 10.2174/1386207323666200903152811.
Eimeriosis is a parasitic intestinal infection that affects the poultry industry; it is responsible for economic losses on a global scale.
This study investigated the protective role of Morus nigra leaf extracts (ME) against Eimeria papillata infection in mice.
C57Bl/6 mice were divided into six groups. The first group was gavaged daily with 100 μL 0.9% NaCl. The second group was treated daily by oral gavage with 100 μL M. nigra leaf extracts (ME) (200 mg/kg), while the third, fourth, fifth, and sixth groups were orally infected with 1000 E. papillata oocysts. The last three groups were daily treated for 5 days with 200, 400, and 800 mg/kg of ME, respectively. Samples of jejunum were obtained for examining the histopathological changes as well as changes in the nitric oxide and catalase levels.
Infrared spectroscopy of ME showed the presence of several expected active classes of phytochemical compounds. ME was able to reduce the E. papillata oocysts in mice feces by about 80% and decrease the infection-induced jejunal histopathological injuries. This was quantified using the histological injury score. In addition, ME significantly recovered the changes in nitric oxide and catalase levels.
Our findings showed that the M. nigra extract has an antioxidant activity which protects against murine eimeriosis. Further studies are required to determine the exact active compounds in ME and their mode of action.
艾美耳球虫病是一种影响家禽业的寄生性肠道感染疾病，在全球范围内造成经济损失。
本研究调查了黑桑叶提取物（ME）对小鼠感染乳头艾美耳球虫的保护作用。
将C57Bl/6小鼠分为六组。第一组每天灌胃100 μL 0.9%氯化钠。第二组每天经口灌胃100 μL黑桑叶提取物（ME）（200 mg/kg），而第三、第四、第五和第六组经口感染1000个乳头艾美耳球虫卵囊。最后三组分别每天用200、400和800 mg/kg的ME治疗5天。获取空肠样本以检查组织病理学变化以及一氧化氮和过氧化氢酶水平的变化。
ME的红外光谱显示存在几种预期的活性植物化学化合物类别。ME能够使小鼠粪便中的乳头艾美耳球虫卵囊减少约80%，并减轻感染引起的空肠组织病理学损伤。这通过组织学损伤评分进行量化。此外，ME显著恢复了一氧化氮和过氧化氢酶水平的变化。
我们的研究结果表明，黑桑提取物具有抗氧化活性，可预防小鼠艾美耳球虫病。需要进一步研究以确定ME中确切的活性化合物及其作用方式。
