Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.
Center of General Education, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
Med Sci Monit. 2020 Sep 4;26:e924411. doi: 10.12659/MSM.924411.
BACKGROUND An innovative animal running wheel with an individualized design was implemented for the rehabilitation of rats following ischemic stroke. MATERIAL AND METHODS The design of the running wheel platform included the running wheel and a side plate for exercise area adjustments. A U-curve with a width of 2 cm was drawn on the lower half of the side plate for the dynamic adjustments of five infrared (IR) sensors based on the physical fitness of the rats. The individualized training process for this running wheel consisted of 2 days of free training to record their average and maximum speeds, 3 days of progressive training to determine their exercise areas, and 2 weeks of normal training based on their average speeds, maximum speeds, and exercise areas. Blood samples were obtained from the tail veins of all rats before the operations and on Days 14, 21, and 28 postsurgery to measure cortisol levels. The motor function tests were performed on Days 7 and 28 postsurgery. On Day 28 postsurgery, the rats were sacrificed under anesthesia, and their brains were removed for Nissl and H&E staining. RESULTS On Day 28 after surgery, the motor function, lesion volume, and cell damage of the DEARW and control groups differed significantly, indicating that this device is effective for stroke rehabilitation. CONCLUSIONS The outcomes of the rats that were rehabilitated using the newly designed training system were better than those of their control-group counterparts, indicating the advantages of this designed system.
为了对缺血性脑卒中后的大鼠进行康复治疗，我们设计了一种具有创新性的、可个性化设计的动物跑轮。
跑轮平台的设计包括跑轮和用于调整运动区域的侧板。侧板的下半部分绘制了一个 2 厘米宽的 U 形曲线，根据大鼠的体能状况，对五个红外（IR）传感器进行动态调整。该跑轮的个性化训练过程包括 2 天的自由训练以记录它们的平均速度和最大速度、3 天的渐进式训练以确定它们的运动区域，以及 2 周的基于平均速度、最大速度和运动区域的正常训练。所有大鼠在手术前和手术后第 14、21 和 28 天从尾静脉取血样以测量皮质醇水平。在手术后第 7 和 28 天进行运动功能测试。手术后第 28 天，大鼠在麻醉下被处死，取出大脑进行尼氏染色和 H&E 染色。
手术后第 28 天，DEARW 组和对照组的运动功能、病灶体积和细胞损伤有显著差异，表明该设备对脑卒中康复有效。
使用新设计的训练系统进行康复治疗的大鼠的结果优于对照组大鼠，表明该设计系统具有优势。
