Cao Chen, Feng Yan, Li Hao, Yang Yang, Song Xuerui, Wang Yingzhe, Zhang Guolin, Dou Wei, Liu Weisheng
Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.
Department of Anatomy and Histology, Lanzhou University School of Basic Medical Sciences, Lanzhou, China.
Talanta. 2020 Nov 1;219:121353. doi: 10.1016/j.talanta.2020.121353. Epub 2020 Jul 8.
Biothiols, as basic biological reactive sulfur species, perform vital actions in many critical physiological processes. Simultaneous detection and direct visualization of intracellular biothiols has great significance to figure out their metabolic mechanisms and cellular functions in living organism. However, it is an enormous challenge to selectively sense biothiols, troubled by their similar chemical structures and properties. In this work, we reported a novel chlorinated coumarin based multi-signal fluorescent probe (CC) for discriminative detection of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) through three different emission channels. CC exhibited favorable sensing properties towards biothiols such as large fluorescence enhancement, low limits of detection (6, 3 and 200 nM for Cys, Hcy and GSH), fast response speed (15 min) and pretty good water solubility. Moreover, it was also utilized for discriminative visualization of endogenous and exogenous Cys, Hcy and GSH in A375 cells and Tcam-2 cells successfully.
生物硫醇作为基本的生物活性硫物种，在许多关键的生理过程中发挥着重要作用。对细胞内生物硫醇进行同时检测和直接可视化，对于弄清楚它们在生物体中的代谢机制和细胞功能具有重要意义。然而，由于生物硫醇具有相似的化学结构和性质，选择性地检测它们是一项巨大的挑战。在这项工作中，我们报道了一种基于新型氯化香豆素的多信号荧光探针（CC），通过三种不同的发射通道对半胱氨酸（Cys）、同型半胱氨酸（Hcy）和谷胱甘肽（GSH）进行鉴别检测。CC对生物硫醇表现出良好的传感特性，如荧光大幅增强、检测限低（Cys、Hcy和GSH分别为6、3和200 nM）、响应速度快（15分钟）以及良好的水溶性。此外，它还成功地用于A375细胞和Tcam-2细胞中内源性和外源性Cys、Hcy和GSH的鉴别可视化。
