Xinjiang Key Laboratory of Solid State Physics and Devices, Xinjiang University, Urumqi 830017, China.
School of Physical Science and Technology, Xinjiang University, Urumqi 830017, China.
Sensors (Basel). 2023 Jun 22;23(13):5814. doi: 10.3390/s23135814.
In this paper, a 2-mercaptobenzimidazole-copper nanoparticles (MBI-CuNPs) fluorescent probe with high performance based on 2-mercaptobenzimidazole functionalized copper nanoparticles was synthesized by a hydrothermal method and used for cysteine (Cys) detection in serum. The MBI-CuNPs probe exhibits strong fluorescence emission at 415 nm under the excitation at 200 nm, which is attributed to the metal-ligand charge transfer (MLCT) transition through the coordination of an MBI ligand and monovalent copper. Furthermore, the MBI-CuNPs probe has a high quenching fluorescence response to Cys, and shows a good linearity relationship with Cys in 0.05-65 µM, with a detection limit of 52 nM. Moreover, the MBI-CuNPs probe could eliminate the interference of biological mercaptan Hcy and GSH with a similar structure and reaction properties, due to the strong electron-donating ability of Cys, which can quench the fluorescence of the MBI-CuNPs probe. The MBI-CuNPs probe was applied to the analysis of Cys in real serum, and the absolute recovery rate was as high as 90.23-97.00%. Such a fluorescent probe with high sensitivity and selectivity has potential applications for the early prevention of various diseases caused by abnormal Cys levels.
本文采用水热法合成了一种基于 2-巯基苯并咪唑功能化铜纳米粒子（MBI-CuNPs）的高性能 2-巯基苯并咪唑-铜纳米粒子（MBI-CuNPs）荧光探针，用于血清中半胱氨酸（Cys）的检测。MBI-CuNPs 探针在 200nm 激发下于 415nm 处表现出强荧光发射，归因于 MBI 配体与单价铜的配位通过金属-配体电荷转移（MLCT）跃迁。此外，MBI-CuNPs 探针对 Cys 具有高猝灭荧光响应，并在 0.05-65µM 范围内表现出良好的线性关系，检测限为 52nM。此外，由于 Cys 具有较强的供电子能力，MBI-CuNPs 探针可以消除具有相似结构和反应性质的生物硫醇 Hcy 和 GSH 的干扰，从而猝灭 MBI-CuNPs 探针的荧光。MBI-CuNPs 探针被应用于实际血清中 Cys 的分析，绝对回收率高达 90.23%-97.00%。这种具有高灵敏度和选择性的荧光探针在早期预防由 Cys 水平异常引起的各种疾病方面具有潜在的应用前景。
