Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel.
Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel.
Talanta. 2020 Nov 1;219:121223. doi: 10.1016/j.talanta.2020.121223. Epub 2020 May 29.
Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption.
需要快速、灵敏的方法来检测致病微生物，以监测牛奶中的污染并确保公众健康。本研究开发了一种基于硝酸纤维素膜的比色抗体堆叠垫系统（生物传感器）来检测牛奶中的微生物含量。该侧向流动免疫分析的改进方法使用了一种多膜生物传感器，从上到下依次为：样品垫、缀合物垫（与辣根过氧化物酶偶联的抗大肠杆菌抗体）、六个大肠杆菌 DH5α 细菌阻断层和带有干燥底物的吸收垫。对有和没有 DH5α 细菌的不同牛奶样品进行了测试。与标准实验室 ELISA 相比，这种多步诊断检测方法的灵敏度提高了 1000 倍以上。这是开发用于在所有加工阶段监测牛奶安全性的简单、便携设备的第一步，目的是确保新鲜、健康的牛奶供人们消费。
